Summary of Study ST002237
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001427. The data can be accessed directly via it's Project DOI: 10.21228/M88D9X This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002237 |
| Study Title | Metabolomic analysis of brain cortex from neuronal specific Depdc5 knockout in fed and fasting state |
| Study Type | Depdc5 KO fed vs. fasted comparison |
| Study Summary | Caloric restriction and acute fasting are known to reduce seizures but through unclear mechanisms. In this study, we demonstrate that mTORC1 signaling is reduced after acute fasting of mice. In neurons, mTORC1 is most sensitive to withdrawal of leucine, arginine, and glutamine, which is dependent on DEPDC5. We performed metabolomic analysis of brain cortex from neuronal specific Depdc5 knockout in fed and fasting state. The Depdc5 neuronal specific knockout mice are resistant to sensing significant fluctuations in brain amino acid levels after fasting. These results establish that acute fasting reduces seizure susceptibility in a DEPDC5-dependent manner. |
| Institute | Northwestern University, Feinberg School of Medicine |
| Department | Medicine |
| Laboratory | Chandel Lab |
| Last Name | Chandel |
| First Name | Navdeep |
| Address | 303 E Superior St, Chicago, Illinois, 60611, USA |
| nav@northwestern.edu | |
| Phone | 3125032549 |
| Submit Date | 2022-07-27 |
| Num Groups | 4 |
| Total Subjects | 40 |
| Num Males | 20 |
| Num Females | 20 |
| Publications | DEPDC5-dependent mTORC1 signaling mechanisms are critical for the anti-seizure effects of acute fasting |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2022-08-15 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR001427 |
| Project DOI: | doi: 10.21228/M88D9X |
| Project Title: | Metabolomic analysis of brain cortex from neuronal specific Depdc5 knockout in fed and fasting states |
| Project Type: | LCMS based comprehensive hydrophilic metabolites profiling |
| Project Summary: | We performed a metabolomic analysis of the brain cortex from neuronal-specific Depdc5 knockout in fed and fasting states. |
| Institute: | Northwestern University Feinberg School of Medicine |
| Department: | Medicine |
| Laboratory: | Chandel Lab |
| Last Name: | Chandel |
| First Name: | Navdeep |
| Address: | 303 E Superior St, Chicago, Illinois, 60611, USA |
| Email: | nav@northwestern.edu |
| Phone: | 3125032549 |
| Funding Source: | NIA |
| Publications: | DEPDC5-dependent mTORC1 signaling mechanisms are critical for the anti-seizure effects of acute fasting |
| Contributors: | Christopher J. Yuskaitis, Jinita B. Modasia, Sandra Schroetter, Leigh-Ana Rossitto, Karenna Groff, Christopher Morici, Divakar S. Mithal, Ram P. Chakrabarty, Navdeep S. Chandel, Brendan D. Manning, Mustafa Sahin |
