Summary of Study ST003703
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002298. The data can be accessed directly via it's Project DOI: 10.21228/M8KR8S This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003703 |
| Study Title | NAD Depletion in Skeletal Muscle does not Compromise Muscle Function or Accelerate Aging |
| Study Summary | NAD is a ubiquitous electron carrier essential for energy metabolism and the posttranslational modification of numerous regulatory proteins. Perturbation of NAD metabolism is considered detrimental to health, with NAD depletion commonly thought to promote aging. However, the extent to which cellular NAD concentration can be decreased without deleterious repercussions is unclear. We generated a mouse model where nicotinamide phosphoribosyltransferase (NAMPT)-mediated NAD+ biosynthesis is disrupted in adult skeletal muscle. The resulting 85% decrease in muscle NAD+ abundance was associated with preserved tissue integrity and functionality, as demonstrated by its unchanged morphology, contractility, and exercise tolerance. This lack of defects was corroborated by intact mitochondrial respiratory capacity and unaffected muscle transcriptomic and proteomic profiles. Furthermore, lifelong NAD depletion did not accelerate muscle aging or impair whole-body metabolism. Collectively, these findings indicate that NAD depletion does not contribute to agerelated declines in skeletal muscle function. |
| Institute | University of Copenhagen |
| Last Name | Treebak |
| First Name | Jonas Thue |
| Address | Blegdamsvej 3B, Mærsk Tårnet, 7. sal 2200 København N. |
| jttreebak@sund.ku.dk | |
| Phone | +4524805398 |
| Submit Date | 2025-02-03 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2025-02-24 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN006075 | AN006076 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Vanquish | Thermo Vanquish |
| Column | Waters ACQUITY UPLC CSH C18 (100 x 2.1 mm, 1.7 µm) | Waters ACQUITY UPLC CSH C18 (100 x 2.1 mm, 1.7 µm) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Bruker timsTOF fleX | Bruker timsTOF fleX |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Intensity | Intensity |
MS:
| MS ID: | MS005782 |
| Analysis ID: | AN006075 |
| Instrument Name: | Bruker timsTOF fleX |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | Lipidomics: Data were acquired in MS-only mode, while MS/MS spectra were obtained exclusively from a pooled sample for annotation. Scan range: 50-1700 CID energy: 40V All data was converted. mzML format using ProteoWizard. Data was annotated in MS-Dial (v. 4.9) against the incorporated lipid-blast database with an MS1/MS2 mass tolerance of 0.005/0.01 Da and a minimum identification score of 70%. The annotated compounds were exported to PCDL manager B.08.00 (Agilent Technologies) to create a database for area extraction in Profinder 10.0 (Agilent Technologies). Features with signals less than 5 times those in blanks or missing in more than 20% of QC samples were removed and signals were QC corrected |
| Ion Mode: | POSITIVE |
| MS ID: | MS005783 |
| Analysis ID: | AN006076 |
| Instrument Name: | Bruker timsTOF fleX |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | Lipidomics: Data were acquired in MS-only mode, while MS/MS spectra were obtained exclusively from a pooled sample for annotation. Scan range: 50-1700 CID energy: 40V All data was converted. mzML format using ProteoWizard. Data was annotated in MS-Dial (v. 4.9) against the incorporated lipid-blast database with an MS1/MS2 mass tolerance of 0.005/0.01 Da and a minimum identification score of 70%. The annotated compounds were exported to PCDL manager B.08.00 (Agilent Technologies) to create a database for area extraction in Profinder 10.0 (Agilent Technologies). Features with signals less than 5 times those in blanks or missing in more than 20% of QC samples were removed and signals were QC corrected |
| Ion Mode: | NEGATIVE |
