Summary of Study ST003600
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002228. The data can be accessed directly via it's Project DOI: 10.21228/M8MZ51 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003600 |
| Study Title | Development of Food Material Source Technology for Future Alternative Meats (Including Cultured Meat) |
| Study Summary | The growing demand for sustainable food sources has accelerated the development of cultured meat as an alternative to traditional meat products. This study aims to predict the safety and nutritional equivalence of cultured meat compared to conventional meat using a comprehensive metabolomics approach. In this study, we conducted a comparative metabolomic analysis of conventional chicken meat, muscle satellite cells, and differentiated cells. Our findings reveal that while the overall metabolic profiles of cultured and original meats are comparable, significant differences are observed in specific metabolites. Notably, metabolites associated with nutrient metabolism and synthesis display substantial variations among the samples. These differences suggest that the nutritional content of cultured meat may differ from that of traditional meat, potentially affecting its dietary value. Despite these differences in metabolic profiles, our analysis indicates that there is no significant impact on the safety of cultured meat itself. The safety of cultured meat remains within acceptable limits, and no adverse health risks were identified in the context of this study. However, the observed variations in nutrient-related metabolites highlight the need for further investigation to fully understand their implications. Our study contributes to the ongoing evaluation of cultured meat as a viable and safe alternative in the pursuit of sustainable food sources. |
| Institute | Sangmyung University |
| Last Name | Ihyeon |
| First Name | Cho |
| Address | Jongno-gu, Seoul, 서울, 03057, South Korea |
| mukuro259@gmail.com | |
| Phone | 01021146997 |
| Submit Date | 2024-11-19 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-12-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN005915 | AN005916 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Vanquish | Thermo Vanquish |
| Column | Agilent Eclipse Plus C18 RRHD (50 × 2.1mm, 1.8um) | Agilent Eclipse Plus C18 RRHD (50 × 2.1mm, 1.8um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | peak area | peak area |
MS:
| MS ID: | MS005633 |
| Analysis ID: | AN005915 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Mass spectrometer parameters were as follows: Full‐MS scans, 100 to 1,000 (Positive mode) and 50 to 500 (Negative mode) m/z of scan range, 70,000 of resolution, 1 × 106 of AGC target, and maximum IT of 100 ms; For MS2 scans, the following parameters were used: 17,500 of resolution, 1 × 105 of AGC target, maximum IT was 200 ms, ±2 m/z of isolation width, and NCE for dd‐MS2 of 30; sheath gas flow rate was 19 (Positive mode) and 5 (Negative mode); aux gas flow rate was 1; spray voltage was 3.80 kV(Positive mode) and −3.40 kV(Negative mode); capillary temp was 320 °C; S-lens RF level was 60.0. Using Compound Discoverer 3.3™ (Thermo Fisher Scientific, Waltham, MA, USA), data analysis is conducted from LC-MS/MS raw files of each sample. Analysis is performed in the 'Untargeted Metabolomics with Statistics Detect Unknowns with ID using online Data Base and mzlogic' mode. Compound identification is carried out using mzCloud (ddMS2, https://www.mzcloud.org/) and ChemSpider (formula or exact mass, http:// www.chemspider.com), followed by ddMS2 data similarity searches for all compounds using mzCloud. According to the Metabolomics Standards Initiative (MSI) criteria, compounds are filtered at Level 2 (< 10 ppm, mzcloud score > 80) and Level 1 (ChemSpider < 5 ppm).12 Metabolites with CV values of area exceeding 30% in QC (Pooled samples) are excluded, and duplicate metabolites are removed based on average area intensity. Based on the qualitative and quantitative analysis results of identified metabolites, statistical analysis is performed using MetaboAnalyst 6.0 (http://www.metaboanalyst.ca). |
| Ion Mode: | POSITIVE |
| MS ID: | MS005634 |
| Analysis ID: | AN005916 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Mass spectrometer parameters were as follows: Full‐MS scans, 100 to 1,000 (Positive mode) and 50 to 500 (Negative mode) m/z of scan range, 70,000 of resolution, 1 × 106 of AGC target, and maximum IT of 100 ms; For MS2 scans, the following parameters were used: 17,500 of resolution, 1 × 105 of AGC target, maximum IT was 200 ms, ±2 m/z of isolation width, and NCE for dd‐MS2 of 30; sheath gas flow rate was 19 (Positive mode) and 5 (Negative mode); aux gas flow rate was 1; spray voltage was 3.80 kV(Positive mode) and −3.40 kV(Negative mode); capillary temp was 320 °C; S-lens RF level was 60.0. Using Compound Discoverer 3.3™ (Thermo Fisher Scientific, Waltham, MA, USA), data analysis is conducted from LC-MS/MS raw files of each sample. Analysis is performed in the 'Untargeted Metabolomics with Statistics Detect Unknowns with ID using online Data Base and mzlogic' mode. Compound identification is carried out using mzCloud (ddMS2, https://www.mzcloud.org/) and ChemSpider (formula or exact mass, http:// www.chemspider.com), followed by ddMS2 data similarity searches for all compounds using mzCloud. According to the Metabolomics Standards Initiative (MSI) criteria, compounds are filtered at Level 2 (< 10 ppm, mzcloud score > 80) and Level 1 (ChemSpider < 5 ppm).12 Metabolites with CV values of area exceeding 30% in QC (Pooled samples) are excluded, and duplicate metabolites are removed based on average area intensity. Based on the qualitative and quantitative analysis results of identified metabolites, statistical analysis is performed using MetaboAnalyst 6.0 (http://www.metaboanalyst.ca). |
| Ion Mode: | NEGATIVE |
