Summary of Study ST002802

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001749. The data can be accessed directly via it's Project DOI: 10.21228/M8N14N This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002802
Study TitleNontargeted Serum Metabolomic Profiling of FXR-null and Wild-type Mice
Study SummaryThis study analyzed the serum metabolome of 10 FXR-knockout and 10 wild-type mice by RPLC-HRMS.
University of North Carolina at Chapel Hill
DepartmentDepartment of Environmental Sciences and Engineering
LaboratoryKun Lu
Last NameHsiao
First NameYun-Chung
Address135 Dauer Dr. MHRC1104
Submit Date2023-07-31
Num Groups2
Total Subjects20
Num Males20
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2023-08-15
Release Version1
Yun-Chung Hsiao Yun-Chung Hsiao application/zip

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Combined analysis:

Analysis ID AN004558
Analysis type MS
Chromatography type Reversed phase
Chromatography system Thermo Vanquish
Column Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um)
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive Orbitrap
Units m/z


MS ID:MS004305
Analysis ID:AN004558
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Comments:The mass spectrometry was set to scan under the positive mode with the sheath gas, auxiliary gas, and sweep gas set to flow rates of 50, 13, and 3 psi, respectively. With the spray voltage set to 3.5 kV, the capillary and auxiliary gas heating temperature were respectively controlled to 263°C and 425°C to fully scan across m/z 70 to 1,000. The resolution was set to 70,000 FWHM (m/z 200). The automatic gain control (AGC) and the maximal injection time (MIT) was set to 2×105 and 50 msec, respectively. Routine mass calibrations were conducted before and after the sample analysis. The samples were blocked-randomized for the injection order. Quality control samples for the serum analytes were prepared by pooling the aliquots of each sample. Method blank samples were prepared for the serum samples by surrogating the biospecimen with water and following the same experimental procedures. If MS/MS spectrums were to be collected, the parallel reaction monitoring (PRM) mode was used, with the isolation width, AGC, and MIT set to 1.2, 3×105 and 100 msec, respectively, at the resolution of 17,500 FWHM (m/z 200).