Summary of Study ST002335

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001498. The data can be accessed directly via it's Project DOI: 10.21228/M83D9C This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002335
Study TitlePathogenic Auxilin mutations affect lipids that are critical to Synaptojanin function
Study SummaryMass spectrometry was performed on heads of 15DO flies homogenized in 100 µl D-PBS (Dulbecco’s phosphate-buffered saline without Mg2+ and Ca2+) by Lipotype. Fifteen fly heads were pooled from three independent crosses for each analysis, and mass spectrometry was performed on n=7 for wild-type, n≥4 analyses for dAuxWT/WT, dAuxRG/RG, dAuxWT/F956x and dAuxRG/F956x and n=3 for dAuxWT/F956x and dAuxRG/F956x overexpressing Synj.
VIB-KU Leuven
Last NameJacquemyn
First NameJulie
AddressMedical Sciences Building 7-25, Edmonton, Alberta, T6H 0L2, Canada
Phone+1 (587) 3409325
Submit Date2022-10-27
Analysis Type DetailLC-MS
Release Date2022-11-21
Release Version1
Julie Jacquemyn Julie Jacquemyn application/zip

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Combined analysis:

Analysis ID AN003814
Analysis type MS
Chromatography type None (Direct infusion)
Chromatography system none
Column none
MS instrument type QTRAP
MS instrument name Thermo Q Exactive Orbitrap
Units pMol


MS ID:MS003556
Analysis ID:AN003814
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:QTRAP
MS Comments:Samples are analyzed in both positive and negative ion modes, with MS resolution Rm/z=200=280000 and MSMS resolution Rm/z=200=17500, in a single acquisition. Acquired data was processed using lipid identification software based on LipidXplorer (1). Data post-processing and normalization were performed by Lipotype using a developed data management system. Data analysis was performed using GraphPad Prism software 9.3.1. Pmol values (obtained by MS) of individual lipid species were transformed into a fraction of the total PA, DAG, PI, PC, PE, and PS lipids in the sample. (1)Herzog, R. et al. Lipidxplorer: A software for consensual cross-platform lipidomics. PLoS ONE 7, e29851 (2012).