Summary of Study ST002124

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001348. The data can be accessed directly via it's Project DOI: 10.21228/M8GD8D This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002124
Study TitleAmino acids and TCA substrates in hematopoietic cells (Part1)
Study SummaryThis study uses [13C,15N] labeled amino acids to study the amino acid consumption and their catabolism into tricarboxylic acid cycle substrates in hematopoietic stem cells, hemopoietic progenitors, and differentiated hematopoietic cells under different conditions, such as homeostasis and proliferation and with drug treatment.
Institute
Sun Yat-sen University
Last NameZhao
First NameMeng
AddressZhongshan 2nd Road
Emailzhaom38@mail.sysu.edu.cn
Phone18138799889
Submit Date2022-04-06
Raw Data AvailableYes
Raw Data File Type(s)cdf
Analysis Type DetailGC-MS
Release Date2022-11-01
Release Version1
Meng Zhao Meng Zhao
https://dx.doi.org/10.21228/M8GD8D
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN003477
Analysis type MS
Chromatography type GC
Chromatography system TRACE 1310
Column DB-35ms
MS Type EI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive Orbitrap
Ion Mode POSITIVE
Units Peak Area

MS:

MS ID:MS003238
Analysis ID:AN003477
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:EI
MS Comments:GC-MS data was acquired on Q-Exactive Orbitrap mass spectrometer (Thermo Fisher) coupled with Gas Chromatograph system TRACE 1310 (Thermo Fisher). Chromatographic separation was performed on a DB-35ms column module (30 m length x 0.25 mm internal diameter, Agilent Technologies). The column temperature was programmed with an initial temperature of 50 °C for 2 min, then ramped at 10°C/min to 325 °C, and maintained for 5 min. The mass range was set as 50-600 m/z, and the resolution was 60,000. The ion source temperature was 300°C with the transfer line temperature of 250°C, and the electron energy was 70 eV with EI source.
Ion Mode:POSITIVE
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