Summary of Study ST001367
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000935. The data can be accessed directly via it's Project DOI: 10.21228/M8TT3R This work is supported by NIH grant, U2C- DK119886. See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001367 |
| Study Title | Malnutrition and Liver Metabolomics Pre-Intervention (part-I) |
| Study Summary | RP-UPLC-FTMS (+/- ion detection) and HILIC-FTMS (+/- ion detection) were conducted on murine livers from healthy (CON) and malnourished (MAL) mice. To examine the impact of gut microbes and malnutrition, data was also collected from a third group (MBG). |
| Institute | University of British Columbia |
| Department | Microbiology and Immunology |
| Laboratory | B. Brett Finlay |
| Last Name | Bauer |
| First Name | Kylynda |
| Address | Michael Smith Laboratories, #301 – 2185 East Mall, University of British Columbia Vancouver, British Columbia Canada, V6T 1Z4 |
| kcbauer@msl.ubc.ca | |
| Phone | (604) 822-2210 |
| Submit Date | 2020-04-20 |
| Num Groups | 3 |
| Total Subjects | 12 |
| Num Females | 12 |
| Analysis Type Detail | LC-MS |
| Release Date | 2020-08-03 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002275 | AN002276 | AN002277 | AN002278 |
|---|---|---|---|---|
| Chromatography ID | CH001673 | CH001673 | CH001674 | CH001674 |
| MS ID | MS002119 | MS002120 | MS002121 | MS002122 |
| Analysis type | MS | MS | MS | MS |
| Chromatography type | Reversed phase | Reversed phase | HILIC | HILIC |
| Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
| Column | Waters BEH C8 (50 x 2.1mm,1.7um) | Waters BEH C8 (50 x 2.1mm,1.7um) | Waters HILIC (100 x 2.1mm,1.8um) | Waters HILIC (100 x 2.1mm,1.8um) |
| MS Type | ESI | ESI | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap | Orbitrap | Orbitrap |
| MS instrument name | Thermo LTQ-Orbitrap Velos Pro | Thermo LTQ-Orbitrap Velos Pro | Thermo LTQ-Orbitrap Velos Pro | Thermo LTQ-Orbitrap Velos Pro |
| Ion Mode | POSITIVE | NEGATIVE | POSITIVE | NEGATIVE |
| Units | Abundance | abundance | Abundance | abundance |
MS:
| MS ID: | MS002119 |
| Analysis ID: | AN002275 |
| Instrument Name: | Thermo LTQ-Orbitrap Velos Pro |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | For relative quantitation, the MS instrument was run in the survey scan mode with FTMS detection at a mass resolution of 60,000 FWHM at m/z 400. The mass scan range was m/z 80 to 1800, with a reference lock-mass for real-time calibration. Two UPLC-FTMS datasets were acquired for each sample, one with positive-ion detection and the other with negative-ion detection. LC-MS/MS data was also acquired from each sample set with collision induced dissociation (CID) at different levels of normalized collision energy (from publication methods). |
| Ion Mode: | POSITIVE |
| MS ID: | MS002120 |
| Analysis ID: | AN002276 |
| Instrument Name: | Thermo LTQ-Orbitrap Velos Pro |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | For relative quantitation, the MS instrument was run in the survey scan mode with FTMS detection at a mass resolution of 60,000 FWHM at m/z 400. The mass scan range was m/z 80 to 1800, with a reference lock-mass for real-time calibration. Two UPLC-FTMS datasets were acquired for each sample, one with positive-ion detection and the other with negative-ion detection. LC-MS/MS data was also acquired from each sample set with collision induced dissociation (CID) at different levels of normalized collision energy (from publication methods). |
| Ion Mode: | NEGATIVE |
| MS ID: | MS002121 |
| Analysis ID: | AN002277 |
| Instrument Name: | Thermo LTQ-Orbitrap Velos Pro |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | The MS instrument was run in the survey scan mode with FTMS detection at a mass resolution of 60,000 FWHM at m/z 400. Two HILIC-FTMS datasets were acquired for each sample, one with positive-ion detection and the other with negative-ion detection. The mass scan range was m/z 80 to 800 (from publication methods). |
| Ion Mode: | POSITIVE |
| MS ID: | MS002122 |
| Analysis ID: | AN002278 |
| Instrument Name: | Thermo LTQ-Orbitrap Velos Pro |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | The MS instrument was run in the survey scan mode with FTMS detection at a mass resolution of 60,000 FWHM at m/z 400. Two HILIC-FTMS datasets were acquired for each sample, one with positive-ion detection and the other with negative-ion detection. The mass scan range was m/z 80 to 800 (from publication methods). |
| Ion Mode: | NEGATIVE |
