Summary of Study ST001143
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000764. The data can be accessed directly via it's Project DOI: 10.21228/M8XH5B This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001143 |
| Study Title | Microbial depletion and ozone exposure - Lung tissue (part I) |
| Study Summary | Global biochemical profiles were determined in lung tissue collected from untreated control mice and mice treated for two weeks with untreated drinking water or water containing an antibiotic cocktail (ampicillin, neomycin, metronidazole, and vancomycin), followed by a three hour exposure to ambient air or ozone (2ppm). Sample collection occurred 24 hours post-ozone exposure. |
| Institute | Harvard School of Public Health |
| Last Name | Shore |
| First Name | Stephanie |
| Address | 677 Huntington Ave |
| sshore@hsph.harvard.edu | |
| Phone | 6174320989 |
| Submit Date | 2019-02-26 |
| Raw Data Available | Yes |
| Analysis Type Detail | GC-MS/LC-MS |
| Release Date | 2019-05-15 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN001882 | AN001883 | AN001884 | AN001885 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | Reversed phase/HILIC | Reversed phase/HILIC | Reversed phase/HILIC | GC |
| Chromatography system | Waters Acquity | Waters Acquity | Waters Acquity | Thermo Trace DSQ |
| Column | Waters Acquity BEH C18 (100 x 2mm,1.7um)/Waters UPLC BEH Amide (2.1x150 mm,1.7um) | Waters Acquity BEH C18 (100 x 2mm,1.7um)/Waters UPLC BEH Amide (2.1x150 mm,1.7um) | Waters Acquity BEH C18 (100 x 2mm,1.7um)/Waters UPLC BEH Amide (2.1x150 mm,1.7um) | 5% diphenyl/95% dimethyl polysiloxane GC column |
| MS Type | ESI | ESI | ESI | EI |
| MS instrument type | Orbitrap | Orbitrap | Orbitrap | Single quadrupole |
| MS instrument name | Thermo Q Exactive Plus Orbitrap | Thermo Q Exactive Plus Orbitrap | Thermo Q Exactive Plus Orbitrap | Thermo Trace DSQ |
| Ion Mode | POSITIVE | NEGATIVE | NEGATIVE | POSITIVE |
| Units | area under the curve | area under the curve | area under the curve | area under the curve |
MS:
| MS ID: | MS001738 |
| Analysis ID: | AN001882 |
| Instrument Name: | Thermo Q Exactive Plus Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Reversed phase LC-MS Positive |
| Ion Mode: | POSITIVE |
| MS ID: | MS001739 |
| Analysis ID: | AN001883 |
| Instrument Name: | Thermo Q Exactive Plus Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Reversed phase LC-MS Negative |
| Ion Mode: | NEGATIVE |
| MS ID: | MS001740 |
| Analysis ID: | AN001884 |
| Instrument Name: | Thermo Q Exactive Plus Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | HILIC LC-MS Negative |
| Ion Mode: | NEGATIVE |
| MS ID: | MS001741 |
| Analysis ID: | AN001885 |
| Instrument Name: | Thermo Trace DSQ |
| Instrument Type: | Single quadrupole |
| MS Type: | EI |
| MS Comments: | GC-MS |
| Ion Mode: | POSITIVE |
