Summary of Study ST004203
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002638. The data can be accessed directly via it's Project DOI: 10.21228/M8NV8F This work is supported by NIH grant, U2C- DK119886. See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST004203 |
| Study Title | Cell state-specific metabolic networks govern ferroptosis versus apoptosis in small cell lung cancer |
| Study Type | LC/MS |
| Study Summary | LC/MS metabolomics was performed on neuroendocrine (ASCL1 high) and non-neuroendocrine (ASCL1 low) small cell lung cancer cells isolated from the RPR2 genetically engineered mouse model (Rb1 flox/flox; Trp53 flox/flox; Rbl2 flox/flox; Hes1GFP). Cancer cell states were separated based on in vivo Hes1 expression, with ASCL1 high neuroendocrine cells enriched as cells with low/no Hes1 expression and ASCL1 low non-neuroendocrine cells as cells with high Hes1. Agilent 1290 Infinity LC system coupled to an Agilent 6545 Q-TOF mass spectrometer with an Agilent Jet Stream Source was used to detect 1296 entities, of which 474 were significantly altered between the two cell states. Pathway analysis revealed differences in purine metabolism, sphingolipid metabolism, tRNA charging, and glutathione-related redox pathways. These data provide insights into metabolic heterogeneity and redox regulation in SCLC cell states. |
| Institute | Stanford University |
| Last Name | Julien |
| First Name | Sage |
| Address | 265 Campus Drive Stanford, CA 94305-5457 |
| julsage@stanford.edu | |
| Phone | 1-650-498-6603 |
| Submit Date | 2025-09-05 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML, d |
| Analysis Type Detail | LC-MS |
| Release Date | 2025-10-10 |
| Release Version | 1 |
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Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Hes1 expression | Sample source |
|---|---|---|---|
| SA484441 | 1157_1 | High Hes1 expression | Mouse tumor cells |
| SA484442 | 1157_2 | High Hes1 expression | Mouse tumor cells |
| SA484443 | 1157_3 | High Hes1 expression | Mouse tumor cells |
| SA484444 | Hes1_neg_1 | Low Hes1 expression | Mouse tumor cells |
| SA484445 | Hes1_neg_2 | Low Hes1 expression | Mouse tumor cells |
| SA484446 | Hes1_neg_3 | Low Hes1 expression | Mouse tumor cells |
| Showing results 1 to 6 of 6 |
