Summary of Study ST003745

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002331. The data can be accessed directly via it's Project DOI: 10.21228/M8B246 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003745
Study TitleScreen potential marker proteins influencing spermatogenesis in the testes of Shandong Black Cattle bulls via multi-omics integrated analysis.
Study SummaryThis study was designed to identify candidate marker proteins that influence the growth and development of Shandong Black Cattle bull testes through a multi-omics joint analysis, thereby providing a certain theoretical basis for testis growth and development as well as bull selection. Eight 12-month-old Shandong Black Cattle bulls were selected, and testes tissues were collected. The testes were categorized into two groups based on their morphological characteristics: Group 1 (weight > 120 g) and Group 2 (weight < 120 g). Group 2 was employed as the control group to construct a protein and metabolite library for joint analysis to screen candidate marker proteins that affect testis growth and development. The results revealed that 1553 differential proteins (DEPs) were differentially expressed between the large and small testes of Black Bleykett bulls, with 1219 being upregulated and 334 being downregulated. The KEGG enrichment results manifested that the upregulated DEPs were primarily involved in the cell cycle (CDK1, CCNB, MCM4), DNA replication (MCM3, MCM4), etc. The downregulated DEPs were mainly associated with metabolic pathways (ACSM1, IMPDH1), etc. The GO enrichment results disclosed that the DEPs were significantly enriched in the categories of cytoskeleton movement. The weighted gene co-expression analysis suggested that the testis weight was significantly correlated with MCM, STRADA, and SEC31B. After integrating the DEPs, a PPI analysis was performed, and 10 key regulatory proteins were identified, including MCM3, MCM4, CDK1, and CDK2. Metabolomics demonstrated that 59 upregulated metabolites were enriched in the glucose metabolism pathway (uridine diphosphate glucose), and 14 downregulated metabolites were significantly enriched in metabolic pathways (hypoxanthine).
Institute
Qingdao Agricultural University
Last NameDONG
First NameYA JUAN
Address700 Changcheng Road, Chengyang District
Emailetcenter@126.com
Phone13561688666
Submit Date2025-02-16
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2025-03-09
Release Version1
YA JUAN DONG YA JUAN DONG
https://dx.doi.org/10.21228/M8B246
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Mammal; Subject species: Bos taurus (Factor headings shown in green)

mb_sample_id local_sample_id Sample source Group
SA408205G01970-2Testis Control
SA408206G01994-2Testis Control
SA408207G01994-1Testis Control
SA408208G06650-2Testis Control
SA408209G06650-1Testis Control
SA408210G06649-2Testis Control
SA408211G06649-1Testis Control
SA408212G06970-2Testis Control
SA408213G06970-1Testis Control
SA408214G01970-1Testis Control
SA408215G01987-2Testis Normal
SA408216G01952-2Testis Normal
SA408217G01987-1Testis Normal
SA408218G06733-2Testis Normal
SA408219G06733-1Testis Normal
SA408220G01977-2Testis Normal
SA408221G01977-1Testis Normal
SA408222G01957-1Testis Normal
SA408223G01957-2Testis Normal
SA408224G01952-6Testis Normal
SA408225G01952-5Testis Normal
SA408226G01952-4Testis Normal
SA408227G01952-3Testis Normal
SA408228G01952-1Testis Normal
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