Summary of Study ST003452

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002126. The data can be accessed directly via it's Project DOI: 10.21228/M8T52T This work is supported by NIH grant, U2C- DK119886. See: https://www.metabolomicsworkbench.org/about/howtocite.php

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files (Contains raw data)
Study IDST003452
Study TitleIntegrated Proteomic and Lipidomic Analysis
Study SummaryGNB1 and SCARB2 genes were knocked down in human subcutaneous adipocytes using RNA interference. Both proteomic and lipidomic analyses were performed using mass spectrometry. The proteomic results revealed significant changes in the expression levels of key proteins involved in lipid metabolism and adipogenesis. The lipidomic analysis identified significant changes in lipid species, including phosphatidylcholines, ceramides, and cholesterol esters. This integrated analysis provides insights into the molecular pathways and lipid metabolism regulated by GNB1 and SCARB2 in adipocyte function.
Institute
Hamamatsu University School of Medicine
Last NameKitamoto
First NameTakuya
Address1-20-1 Handayama, Chuo-ku, Hamamatsu 431-3192, Japan
Emailt.ktmt@hama-med.ac.jp
Phone+81-53-435-2987
Submit Date2024-08-05
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2024-12-27
Release Version1
Takuya Kitamoto Takuya Kitamoto
https://dx.doi.org/10.21228/M8T52T
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Factors:

Subject type: Cultured cells; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Gene Knockdown Target
SA380956lipidome_GNB1_3GNB1
SA380957proteome_GNB1_1GNB1
SA380958proteome_GNB1_2GNB1
SA380959proteome_GNB1_3GNB1
SA380960lipidome_GNB1_2GNB1
SA380961lipidome_GNB1_1GNB1
SA380962lipidome_nc_2Negative Control
SA380963lipidome_nc_3Negative Control
SA380964proteome_nc_1Negative Control
SA380965proteome_nc_2Negative Control
SA380966proteome_nc_3Negative Control
SA380967lipidome_nc_1Negative Control
SA380968proteome_SCARB2_3SCARB2
SA380969proteome_SCARB2_2SCARB2
SA380970proteome_SCARB2_1SCARB2
SA380971lipidome_SCARB2_1SCARB2
SA380972lipidome_SCARB2_2SCARB2
SA380973lipidome_SCARB2_3SCARB2
Showing results 1 to 18 of 18
  logo