Summary of Study ST003266

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002028. The data can be accessed directly via it's Project DOI: 10.21228/M8GC01 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003266
Study TitleMetabolomics analysis of human spermatozoa reveals impaired metabolic pathways in asthenozoospermia (MS data)
Study SummaryBackground: Infertility is a major health issue, affecting 15% of reproductive-age couples with male factors contributing to 50% of cases. Asthenozoospermia, or low sperm motility, is a common cause of male infertility with complex etiology, involving genetic and metabolic alterations, inflammation, and oxidative stress. However, the molecular mechanisms behind low motility are unclear. In this study, we used a metabolomics approach to identify metabolic biomarkers and pathways involved in sperm motility. Methods: We compared the metabolome and lipidome of spermatozoa of men with normozoospermia (n = 44) and asthenozoospermia (n = 22) using untargeted LC-MS and the metabolome of seminal fluid using 1H-NMR. Additionally, we evaluated the seminal fluid redox status to assess the oxidative stress in the ejaculate. Results: We identified 112 metabolites and 209 lipids in spermatozoa and 27 metabolites in the seminal fluid of normozoospermic and asthenozoospermic men. PCA analysis of the spermatozoa’s metabolomics and lipidomics data showed a clear separation between groups. Spermatozoa of asthenozoospermic men presented lower levels of several amino acids, and increased levels of energetic substrates and lysophospholipids. However, the metabolome and redox status of the seminal fluid was not altered in asthenozoospermia. Conclusions: Our results indicate impaired metabolic pathways associated with redox homeostasis and amino acid, energy, and lipid metabolism in asthenozoospermia. Taken together, these findings suggest that the metabolome and lipidome of human spermatozoa are key factors influencing their motility and that oxidative stress exposure during spermatogenesis or sperm maturation may be in the etiology of decreased motility in asthenozoospermia.
Institute
University of Aveiro
DepartmentDepartment of Chemistry
Last NameGuerra-Carvalho
First NameBárbara
AddressCampus Universitário de Santiago, 3810-193 Aveiro, Portugal
Emailbarbaraggcarvalho@gmail.com
Phone234 370 360
Submit Date2024-06-15
Num Groups2
Total Subjects57
Num Males57
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2024-07-25
Release Version1
Bárbara Guerra-Carvalho Bárbara Guerra-Carvalho
https://dx.doi.org/10.21228/M8GC01
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Group Sample source
SA354284Not applicable2Asthenozoospermia Pooled spermatozoa
SA354285Not applicable1Asthenozoospermia Pooled spermatozoa
SA354286N49Asthenozoospermia Spermatozoa
SA354287N15Asthenozoospermia Spermatozoa
SA354288N64Asthenozoospermia Spermatozoa
SA354289N58Asthenozoospermia Spermatozoa
SA354290N56Asthenozoospermia Spermatozoa
SA354291N54Asthenozoospermia Spermatozoa
SA354292N53Asthenozoospermia Spermatozoa
SA354293N14Asthenozoospermia Spermatozoa
SA354294N44Asthenozoospermia Spermatozoa
SA354295N28Asthenozoospermia Spermatozoa
SA354296N43Asthenozoospermia Spermatozoa
SA354297N26Asthenozoospermia Spermatozoa
SA354298N17Asthenozoospermia Spermatozoa
SA354299N29Asthenozoospermia Spermatozoa
SA354300N3Asthenozoospermia Spermatozoa
SA354301N34Asthenozoospermia Spermatozoa
SA354302N4Asthenozoospermia Spermatozoa
SA354303N27Asthenozoospermia Spermatozoa
SA354304Not applicable4Normozoospermia Pooled spermatozoa
SA354305Not applicable3Normozoospermia Pooled spermatozoa
SA354306N60Normozoospermia Spermatozoa
SA354307N51Normozoospermia Spermatozoa
SA354308N52Normozoospermia Spermatozoa
SA354309N57Normozoospermia Spermatozoa
SA354310N59Normozoospermia Spermatozoa
SA354311N6Normozoospermia Spermatozoa
SA354312N7Normozoospermia Spermatozoa
SA354313N61Normozoospermia Spermatozoa
SA354314N62Normozoospermia Spermatozoa
SA354315N68Normozoospermia Spermatozoa
SA354316N69Normozoospermia Spermatozoa
SA354317N5Normozoospermia Spermatozoa
SA354318N70Normozoospermia Spermatozoa
SA354319N8Normozoospermia Spermatozoa
SA354320N9Normozoospermia Spermatozoa
SA354321N50Normozoospermia Spermatozoa
SA354322N42Normozoospermia Spermatozoa
SA354323N48Normozoospermia Spermatozoa
SA354324N20Normozoospermia Spermatozoa
SA354325N10Normozoospermia Spermatozoa
SA354326N11Normozoospermia Spermatozoa
SA354327N12Normozoospermia Spermatozoa
SA354328N13Normozoospermia Spermatozoa
SA354329N16Normozoospermia Spermatozoa
SA354330N18Normozoospermia Spermatozoa
SA354331N19Normozoospermia Spermatozoa
SA354332N2Normozoospermia Spermatozoa
SA354333N21Normozoospermia Spermatozoa
SA354334N47Normozoospermia Spermatozoa
SA354335N23Normozoospermia Spermatozoa
SA354336N24Normozoospermia Spermatozoa
SA354337N25Normozoospermia Spermatozoa
SA354338N35Normozoospermia Spermatozoa
SA354339N37Normozoospermia Spermatozoa
SA354340N38Normozoospermia Spermatozoa
SA354341N41Normozoospermia Spermatozoa
SA354342N45Normozoospermia Spermatozoa
SA354343N46Normozoospermia Spermatozoa
SA354344N30Normozoospermia Spermatozoa
Showing results 1 to 61 of 61
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