Summary of Study ST003090
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001919. The data can be accessed directly via it's Project DOI: 10.21228/M8P13K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003090 |
| Study Title | Analysis of lipid profiles of N2A-Pz1-KO cells expressing tdTOMATO-vector (mock), TMEM120A, or TMEM120B |
| Study Type | Lipidomic |
| Study Summary | N2A-Pz1-KO cells expressing tdTOMATO-vector (mock), TMEM120A, or TMEM120B were used to detect differences in levels of lipids. TMEM120A is a protein without a clear function that we previously identified to inhibit mechanically activated PIEZO2 channels. TMEM120A has structural similarity a lipid modifying enzyme (ELOVL7), and has been proposed to be necessary for adipocyte maturation & triglyceride production. We performed LC-MS/MS to determine whether TMEM120A can modify the lipid content of cells where it is expressed and utilize that data to assess whether those lipids can inhibit PIEZO2 channels. This study revealed that TMEM120A expressing cells do have larger levels of phosphatidic acid and lysophosphatidic acid, and subsequent functional studies revealed that these lipids do inhibit PIEZO2. |
| Institute | Rutgers University |
| Department | Pharmacology, Physiology and Neuroscience; Metabolomics Shared Resource |
| Laboratory | Tibor Rohacs; Xiaoyang Su |
| Last Name | Rohacs |
| First Name | Tibor |
| Address | 183 South Orange Ave, Newark, NJ, 07103 |
| rohacsti@njms.rutgers.edu | |
| Phone | 973-972-4464 |
| Submit Date | 2024-02-01 |
| Num Groups | 3 |
| Total Subjects | 9 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-07-16 |
| Release Version | 1 |
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Factors:
Subject type: Cultured cells; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Sample source | Transfection |
|---|---|---|---|
| SA332976 | Mock_1 | Neuro 2A cells | Mock |
| SA332977 | Mock_3 | Neuro 2A cells | Mock |
| SA332978 | Mock_2 | Neuro 2A cells | Mock |
| SA332979 | TMEM120A_3 | Neuro 2A cells | TMEM120A |
| SA332980 | TMEM120A_2 | Neuro 2A cells | TMEM120A |
| SA332981 | TMEM120A_1 | Neuro 2A cells | TMEM120A |
| SA332982 | TMEM120B_3 | Neuro 2A cells | TMEM120B |
| SA332983 | TMEM120B_2 | Neuro 2A cells | TMEM120B |
| SA332984 | TMEM120B_1 | Neuro 2A cells | TMEM120B |
| Showing results 1 to 9 of 9 |
