Summary of Study ST003069
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001899. The data can be accessed directly via it's Project DOI: 10.21228/M88147 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003069 |
| Study Title | Plasma instead of serum avoids critical confounding of clinical metabolomics studies by platelets (Part 3/3 - Plasma and serum metabolomics) |
| Study Summary | Metabolomics is an emerging and powerful molecular profiling method supporting clinical investigations. Serum and plasma are commonly used without rational prioritization. Serum is collected after blood coagulation, a complex biochemical process involving active platelet metabolism. This may affect the metabolome and increase the variance as platelet counts and function may vary substantially in individuals. A multi-omics approach systematically investigating the suitability of serum and plasma for clinical studies demonstrated that metabolites correlated well (n=461, R2=0.991), whereas lipid mediators (n=104, R2=0.906) and proteins (n=322, R2=0.860) differed substantially between specimen. Independently, analysis of platelet releasates identified most biomolecules significantly enriched in serum when compared to plasma. A prospective, randomized, controlled parallel group metabolomics trial with acetylsalicylic acid administered for 7 days demonstrated that the apparent drug effects significantly differ depending on analyzed specimen. Only serum analyses of healthy individuals suggested a significant downregulation of TXB2 and 12-HETE, which were specifically formed during coagulation in vitro. Plasma analyses reliably identified acetylsalicylic acid effects on metabolites and lipids occurring in vivo such as a decrease in polyunsaturated fatty acids. The present data suggests that plasma should be preferred above serum for clinical metabolomics studies as the serum metabolome may be substantially confounded by platelets. |
| Institute | University of Vienna |
| Department | Department of Analytical Chemistry |
| Laboratory | Gerner lab |
| Last Name | Meier-Menches |
| First Name | Samuel |
| Address | Währingerstraße 38, 1090 Vienna, Austria |
| samuel.meier-menches@univie.ac.at | |
| Phone | +43-1-4277-52373 |
| Submit Date | 2024-01-22 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-04-12 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Sample material | Treatment | Sex |
|---|---|---|---|---|
| SA332108 | MXP500L-0-5711_1036867631_89_1_1_1_00_1036867083_6_R24V3 | Plasma | after_Aspirin | F |
| SA332109 | MXP500L-0-5711_1036867631_71_1_1_1_00_1036867050_3_R22V3 | Plasma | after_Aspirin | F |
| SA332110 | MXP500L-0-5711_1036867631_10_1_1_1_00_1036866394_12_R28V3 | Plasma | after_Aspirin | F |
| SA332111 | MXP500L-0-5711_1036867631_19_1_1_1_00_1036866439_16_R30V3 | Plasma | after_Aspirin | F |
| SA332112 | MXP500L-0-5711_1036867631_89_0_1_1_00_1036867083_6_R24V3 | Plasma | after_Aspirin | F |
| SA332113 | MXP500L-0-5711_1036867631_71_0_1_1_00_1036867050_3_R22V3 | Plasma | after_Aspirin | F |
| SA332114 | MXP500L-0-5711_1036867631_10_0_1_1_00_1036866394_12_R28V3 | Plasma | after_Aspirin | F |
| SA332115 | MXP500L-0-5711_1036867631_19_0_1_1_00_1036866439_16_R30V3 | Plasma | after_Aspirin | F |
| SA332116 | MXP500L-0-5711_1036867631_40_1_1_1_00_1036867113_9_R26V3 | Plasma | after_Aspirin | M |
| SA332117 | MXP500L-0-5711_1036867631_40_0_1_1_00_1036867113_9_R26V3 | Plasma | after_Aspirin | M |
| SA332118 | MXP500L-0-5711_1036867631_60_0_1_1_00_1036866410_14_R29V3 | Plasma | after_Aspirin | M |
| SA332119 | MXP500L-0-5711_1036867631_60_1_1_1_00_1036866410_14_R29V3 | Plasma | after_Aspirin | M |
| SA332120 | MXP500L-0-5711_1036867631_72_1_1_1_00_1036866380_11_R28V2 | Plasma | before_Aspirin | F |
| SA332121 | MXP500L-0-5711_1036867631_66_0_1_1_00_1036867045_2_R22V2 | Plasma | before_Aspirin | F |
| SA332122 | MXP500L-0-5711_1036867631_52_1_1_1_00_1036866424_15_R30V2 | Plasma | before_Aspirin | F |
| SA332123 | MXP500L-0-5711_1036867631_42_1_1_1_00_1036867079_5_R24V2 | Plasma | before_Aspirin | F |
| SA332124 | MXP500L-0-5711_1036867631_66_1_1_1_00_1036867045_2_R22V2 | Plasma | before_Aspirin | F |
| SA332125 | MXP500L-0-5711_1036867631_72_0_1_1_00_1036866380_11_R28V2 | Plasma | before_Aspirin | F |
| SA332126 | MXP500L-0-5711_1036867631_42_0_1_1_00_1036867079_5_R24V2 | Plasma | before_Aspirin | F |
| SA332127 | MXP500L-0-5711_1036867631_52_0_1_1_00_1036866424_15_R30V2 | Plasma | before_Aspirin | F |
| SA332128 | MXP500L-0-5711_1036867631_46_1_1_1_00_1036866405_13_R29V2 | Plasma | before_Aspirin | M |
| SA332129 | MXP500L-0-5711_1036867631_46_0_1_1_00_1036866405_13_R29V2 | Plasma | before_Aspirin | M |
| SA332130 | MXP500L-0-5711_1036867631_94_0_1_1_00_1036867109_8_R26V2 | Plasma | before_Aspirin | M |
| SA332131 | MXP500L-0-5711_1036867631_94_1_1_1_00_1036867109_8_R26V2 | Plasma | before_Aspirin | M |
| SA332096 | MXP500L-0-5711_1036867631_66_0_1_1_00_1036867045_2_D1 | Plasma | Plasma | F |
| SA332097 | MXP500L-0-5711_1036867631_42_1_1_1_00_1036867079_5_D2 | Plasma | Plasma | F |
| SA332098 | MXP500L-0-5711_1036867631_66_1_1_1_00_1036867045_2_D1 | Plasma | Plasma | F |
| SA332099 | MXP500L-0-5711_1036867631_72_1_1_1_00_1036866380_11_D4 | Plasma | Plasma | F |
| SA332100 | MXP500L-0-5711_1036867631_52_0_1_1_00_1036866424_15_D6 | Plasma | Plasma | F |
| SA332101 | MXP500L-0-5711_1036867631_42_0_1_1_00_1036867079_5_D2 | Plasma | Plasma | F |
| SA332102 | MXP500L-0-5711_1036867631_52_1_1_1_00_1036866424_15_D6 | Plasma | Plasma | F |
| SA332103 | MXP500L-0-5711_1036867631_72_0_1_1_00_1036866380_11_D4 | Plasma | Plasma | F |
| SA332104 | MXP500L-0-5711_1036867631_46_0_1_1_00_1036866405_13_D5 | Plasma | Plasma | M |
| SA332105 | MXP500L-0-5711_1036867631_67_1_1_1_00_1036867128_10_D3 | Plasma | Plasma | M |
| SA332106 | MXP500L-0-5711_1036867631_46_1_1_1_00_1036866405_13_D5 | Plasma | Plasma | M |
| SA332107 | MXP500L-0-5711_1036867631_67_0_1_1_00_1036867128_10_D3 | Plasma | Plasma | M |
| SA332144 | MXP500L-0-5711_1036867631_17_1_1_1_00_1036865856_34_R30V3 | Serum | after_Aspirin | F |
| SA332145 | MXP500L-0-5711_1036867631_30_0_1_1_00_1036866481_21_R22V3 | Serum | after_Aspirin | F |
| SA332146 | MXP500L-0-5711_1036867631_45_1_1_1_00_1036865818_30_R28V3 | Serum | after_Aspirin | F |
| SA332147 | MXP500L-0-5711_1036867631_58_1_1_1_00_1036866511_24_R24V3 | Serum | after_Aspirin | F |
| SA332148 | MXP500L-0-5711_1036867631_45_0_1_1_00_1036865818_30_R28V3 | Serum | after_Aspirin | F |
| SA332149 | MXP500L-0-5711_1036867631_30_1_1_1_00_1036866481_21_R22V3 | Serum | after_Aspirin | F |
| SA332150 | MXP500L-0-5711_1036867631_17_0_1_1_00_1036865856_34_R30V3 | Serum | after_Aspirin | F |
| SA332151 | MXP500L-0-5711_1036867631_58_0_1_1_00_1036866511_24_R24V3 | Serum | after_Aspirin | F |
| SA332152 | MXP500L-0-5711_1036867631_06_1_1_1_00_1036866545_27_R26V3 | Serum | after_Aspirin | M |
| SA332153 | MXP500L-0-5711_1036867631_06_0_1_1_00_1036866545_27_R26V3 | Serum | after_Aspirin | M |
| SA332154 | MXP500L-0-5711_1036867631_33_1_1_1_00_1036865837_32_R29V3 | Serum | after_Aspirin | M |
| SA332155 | MXP500L-0-5711_1036867631_33_0_1_1_00_1036865837_32_R29V3 | Serum | after_Aspirin | M |
| SA332156 | MXP500L-0-5711_1036867631_05_0_1_1_00_1036865841_33_R30V2 | Serum | before_Aspirin | F |
| SA332157 | MXP500L-0-5711_1036867631_93_0_1_1_00_1036866564_29_R28V2 | Serum | before_Aspirin | F |
| SA332158 | MXP500L-0-5711_1036867631_82_1_1_1_00_1036866477_20_R22V2 | Serum | before_Aspirin | F |
| SA332159 | MXP500L-0-5711_1036867631_48_0_1_1_00_1036866507_23_R24V2 | Serum | before_Aspirin | F |
| SA332160 | MXP500L-0-5711_1036867631_93_1_1_1_00_1036866564_29_R28V2 | Serum | before_Aspirin | F |
| SA332161 | MXP500L-0-5711_1036867631_48_1_1_1_00_1036866507_23_R24V2 | Serum | before_Aspirin | F |
| SA332162 | MXP500L-0-5711_1036867631_05_1_1_1_00_1036865841_33_R30V2 | Serum | before_Aspirin | F |
| SA332163 | MXP500L-0-5711_1036867631_82_0_1_1_00_1036866477_20_R22V2 | Serum | before_Aspirin | F |
| SA332164 | MXP500L-0-5711_1036867631_95_0_1_1_00_1036866531_26_R26V2 | Serum | before_Aspirin | M |
| SA332165 | MXP500L-0-5711_1036867631_95_1_1_1_00_1036866531_26_R26V2 | Serum | before_Aspirin | M |
| SA332166 | MXP500L-0-5711_1036867631_24_1_1_1_00_1036865822_31_R29V2 | Serum | before_Aspirin | M |
| SA332167 | MXP500L-0-5711_1036867631_24_0_1_1_00_1036865822_31_R29V2 | Serum | before_Aspirin | M |
| SA332132 | MXP500L-0-5711_1036867631_05_1_1_1_00_1036865841_33_D6 | Serum | Serum | F |
| SA332133 | MXP500L-0-5711_1036867631_93_0_1_1_00_1036866564_29_D4 | Serum | Serum | F |
| SA332134 | MXP500L-0-5711_1036867631_48_0_1_1_00_1036866507_23_D2 | Serum | Serum | F |
| SA332135 | MXP500L-0-5711_1036867631_82_0_1_1_00_1036866477_20_D1 | Serum | Serum | F |
| SA332136 | MXP500L-0-5711_1036867631_82_1_1_1_00_1036866477_20_D1 | Serum | Serum | F |
| SA332137 | MXP500L-0-5711_1036867631_05_0_1_1_00_1036865841_33_D6 | Serum | Serum | F |
| SA332138 | MXP500L-0-5711_1036867631_48_1_1_1_00_1036866507_23_D2 | Serum | Serum | F |
| SA332139 | MXP500L-0-5711_1036867631_93_1_1_1_00_1036866564_29_D4 | Serum | Serum | F |
| SA332140 | MXP500L-0-5711_1036867631_24_1_1_1_00_1036865822_31_D5 | Serum | Serum | M |
| SA332141 | MXP500L-0-5711_1036867631_07_0_1_1_00_1036866550_28_D3 | Serum | Serum | M |
| SA332142 | MXP500L-0-5711_1036867631_07_1_1_1_00_1036866550_28_D3 | Serum | Serum | M |
| SA332143 | MXP500L-0-5711_1036867631_24_0_1_1_00_1036865822_31_D5 | Serum | Serum | M |
| Showing results 1 to 72 of 72 |
