Summary of Study ST002349

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001509. The data can be accessed directly via it's Project DOI: 10.21228/M8N71K This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files (Contains raw data)
Study IDST002349
Study TitleBiomolecular condensates create phospholipid-enriched microenvironments (Part 1)
Study TypeMetabolomes of in vitro synthesized condensates
Study SummaryProteins and RNA are able to phase separate from the aqueous cellular environment to form sub-cellular compartments called condensates. This process results in a protein-RNA mixture that is chemically distinct from the surrounding aqueous phase. Here we use mass spectrometry to characterize the metabolomes of condensates. To test this, we prepared mixtures of phase-separated proteins and cellular metabolites and identified metabolites enriched in the condensate phase. These proteins included SARS-CoV-2 nucleocapsid, as well as low complexity domains of MED1 and HNRNPA1.
Institute
Cornell University
DepartmentDepartment of Pharmacology
LaboratoryDr. Samie Jaffrey
Last NameDumelie
First NameJason
Address1300 York Ave, LC-524, New York City, NY
Emailjdumes98@gmail.com
Phone6465690174
Submit Date2022-11-04
Raw Data AvailableYes
Raw Data File Type(s)mzdata.xml
Analysis Type DetailOther
Release Date2023-03-01
Release Version2
Jason Dumelie Jason Dumelie
https://dx.doi.org/10.21228/M8N71K
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Protein RNA Fraction
SA249704HNRNPA1 Aqueous Sample 2HNRNPA1 150 nM aqueous
SA249705HNRNPA1 Aqueous Sample 3HNRNPA1 150 nM aqueous
SA249706HNRNPA1 Aqueous Sample 1HNRNPA1 150 nM aqueous
SA249707HNRNPA1 Condensate Sample 1HNRNPA1 150 nM condensate
SA249708HNRNPA1 Condensate Sample 3HNRNPA1 150 nM condensate
SA249709HNRNPA1 Condensate Sample 2HNRNPA1 150 nM condensate
SA249710HNRNPA1 Input Sample 2HNRNPA1 150 nM input
SA249711HNRNPA1 Input Sample 1HNRNPA1 150 nM input
SA249712HNRNPA1 Input Sample 3HNRNPA1 150 nM input
SA249713MED1 Aqueous Sample 1MED1 150 nM aqueous
SA249714MED1 Aqueous Sample 3MED1 150 nM aqueous
SA249715MED1 Aqueous Sample 2MED1 150 nM aqueous
SA249716MED1 Condensate Sample 1MED1 150 nM condensate
SA249717MED1 Condensate Sample 3MED1 150 nM condensate
SA249718MED1 Condensate Sample 2MED1 150 nM condensate
SA249719MED1 Input Sample 3MED1 150 nM input
SA249720MED1 Input Sample 2MED1 150 nM input
SA249721MED1 Input Sample 1MED1 150 nM input
SA249722Nucleocapsid No RNA Aqueous Sample 4SARA CoV-2 nucleocapsid 0 nM aqueous
SA249723Nucleocapsid No RNA Aqueous Sample 5SARA CoV-2 nucleocapsid 0 nM aqueous
SA249724Nucleocapsid No RNA Condensate Sample 5SARA CoV-2 nucleocapsid 0 nM condensate
SA249725Nucleocapsid No RNA Condensate Sample 4SARA CoV-2 nucleocapsid 0 nM condensate
SA249726Nucleocapsid No RNA Input Sample 4SARA CoV-2 nucleocapsid 0 nM input
SA249727Nucleocapsid No RNA Input Sample 5SARA CoV-2 nucleocapsid 0 nM input
SA249728Nucleocapsid Aqueous Sample 1SARA CoV-2 nucleocapsid 150 nM aqueous
SA249729Nucleocapsid Aqueous Sample 2SARA CoV-2 nucleocapsid 150 nM aqueous
SA249730Nucleocapsid Aqueous Sample 3SARA CoV-2 nucleocapsid 150 nM aqueous
SA249731Nucleocapsid Condensate Sample 1SARA CoV-2 nucleocapsid 150 nM condensate
SA249732Nucleocapsid Condensate Sample 3SARA CoV-2 nucleocapsid 150 nM condensate
SA249733Nucleocapsid Condensate Sample 2SARA CoV-2 nucleocapsid 150 nM condensate
SA249734Nucleocapsid Input Sample 2SARA CoV-2 nucleocapsid 150 nM input
SA249735Nucleocapsid Input Sample 1SARA CoV-2 nucleocapsid 150 nM input
SA249736Nucleocapsid Input Sample 3SARA CoV-2 nucleocapsid 150 nM input
SA249737Nucleocapsid 600 nM RNA Aqueous Sample 7SARA CoV-2 nucleocapsid 600 nM aqueous
SA249738Nucleocapsid 600 nM RNA Aqueous Sample 6SARA CoV-2 nucleocapsid 600 nM aqueous
SA249739Nucleocapsid 600 nM RNA Condensate Sample 7SARA CoV-2 nucleocapsid 600 nM condensate
SA249740Nucleocapsid 600 nM RNA Condensate Sample 6SARA CoV-2 nucleocapsid 600 nM condensate
SA249741Nucleocapsid 600 nM RNA Input Sample 6SARA CoV-2 nucleocapsid 600 nM input
SA249742Nucleocapsid 600 nM RNA Input Sample 7SARA CoV-2 nucleocapsid 600 nM input
Showing results 1 to 39 of 39
  logo