Summary of Study ST002270
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001452. The data can be accessed directly via it's Project DOI: 10.21228/M81Q5B This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002270 |
| Study Title | Xenopus tropicalis glycolysis and PPP inhibition |
| Study Summary | Stage 41 tadpoles were injected with 4 nmol of the glycolysis inhibitor, 2-deoxyglucose (2DG), or a tracer control to evaluate consequences of inhibition on metabolites 24 hours after treatment began. Similarly, tadpoles were incubated in DMSO control or 1 of 2 G6PD inhibitors (Dehydroepiandrosterone and g6pdi), to similarly assess the consequences of inhibiting the pentose phosphate pathway. We find that inhibition of glucose metabolism with 2DG results in a decrease in downstream glycolytic intermediates, confirming a reduction in activity of this pathway. G6PD inhibition was not as clear as changes were less consistent across treatments and downstream metabolites did not behave in a coordinated way, though impacts on other metabolic processes by these inhibitors may be fruitful for exploring how they perturb metabolism in the tadpoles. |
| Institute | University of Washington |
| Last Name | Patel |
| First Name | Jeet |
| Address | 1705 NE Pacific St., HSB J-Wing, J405, Seattle, Washington, 98195, USA |
| pateljeet1224@gmail.com | |
| Phone | 2065431748 |
| Submit Date | 2022-08-03 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2022-10-25 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Factors:
Subject type: Other organism; Subject species: Xenopus tropicalis (Factor headings shown in green)
| mb_sample_id | local_sample_id | Condition |
|---|---|---|
| SA217736 | 2DG2 | 2DG |
| SA217737 | 2DG4 | 2DG |
| SA217738 | 2DG3 | 2DG |
| SA217739 | 2DG1 | 2DG |
| SA217740 | DHEA2 | DHEA |
| SA217741 | DHEA3 | DHEA |
| SA217742 | DHEA4 | DHEA |
| SA217743 | DHEA1 | DHEA |
| SA217744 | DMSO2 | DMSO |
| SA217745 | DMSO4 | DMSO |
| SA217746 | DMSO3 | DMSO |
| SA217747 | DMSO1 | DMSO |
| SA217752 | g6pdi1 | g6pdi |
| SA217753 | g6pdi2 | g6pdi |
| SA217754 | g6pdi3 | g6pdi |
| SA217755 | g6pdi4 | g6pdi |
| SA217748 | MR3 | MR |
| SA217749 | MR2 | MR |
| SA217750 | MR4 | MR |
| SA217751 | MR1 | MR |
| Showing results 1 to 20 of 20 |
