Summary of Study ST002174

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001383. The data can be accessed directly via it's Project DOI: 10.21228/M8Z993 This work is supported by NIH grant, U2C- DK119886.


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Study IDST002174
Study TitleIdentifying a tryptophan derivative in hydrogen peroxide-treated cell culture medium
Study SummaryReactive oxygen species (ROS) are by-products of metabolism of oxygen and they play an important role in normal homeostasis and cell signaling, as well as in the initiation of diseases including cancer when their production is upregulated. Thus, it is imperative to understand the cellular and molecular basis by which ROS impact on various biological and pathological processes. Here, we identified 2-oxindole, a tryptophan derivative, was a major catabolic product in hydrogen peroxide-treated cell culture medium. We used 2-oxindole to study its role in regulating AhR signaling and tryptophan metabolic pathways. We found that 2-oxindole significantly increased the activity of AhR, leading to enhanced expression of its downstream targets including cytochrome P450 genes.
NYU Langone Health
DepartmentDepartment of Biochemistry and Molecular Pharmacology
Last NameChoi
First NameByeong Hyeok
Address341 East 25 Street New York, NY 10010
Submit Date2022-04-06
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailLC-MS
Release Date2022-06-01
Release Version1
Byeong Hyeok Choi Byeong Hyeok Choi application/zip

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Subject type: Other abiotic sample; Subject species: - (Factor headings shown in green)

mb_sample_id local_sample_id Group
SA20895719 +DON 15N 1DON
SA20895819 +DON 15N 2DON
SA20895919 +DON 15N 3DON
SA20895419 +DON 13C 3+DON
SA20895519 +DON 13C 2+DON
SA20895619 +DON 13C 1+DON
SA20896019 NT 15N 3NT
SA20896119 NT 15N 1NT
SA20896219 NT 15N 2NT
SA20896319 NT 13C 2VEH
SA20896419 NT 13C 1VEH
SA20896519 NT 13C 3VEH
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