Summary of Study ST001709

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001094. The data can be accessed directly via it's Project DOI: 10.21228/M89394 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001709
Study TitleSARS-CoV-2 infection rewires host cell metabolism and is potentially susceptible to mTORC1 inhibition
Study SummaryViruses hijack host cell metabolism to acquire the building blocks required for viral replication. Understanding how SARS-CoV-2 alters host cell metabolism could lead to potential treatments for COVID-19, the disease caused by SARS-CoV-2 infection. Here we profile metabolic changes conferred by SARS-CoV-2 infection in kidney epithelial cells and lung air-liquid interface cultures and show that SARS-CoV-2 infection increases glucose carbon entry into the TCA cycle via increased pyruvate carboxylase expression. SARS-CoV-2 also reduces host cell oxidative glutamine metabolism while maintaining reductive carboxylation. Consistent with these changes in host cell metabolism, we show that SARS-CoV-2 increases activity of mTORC1, a master regulator of anabolic metabolism, in cell lines and patient lung stem cell-derived airway epithelial cells. We also show evidence of mTORC1 activation in COVID-19 patient lung tissue. Notably, mTORC1 inhibitors reduce viral replication in kidney epithelial cells and patient-derived lung stem cell cultures. This suggests that targeting mTORC1 could be a useful antiviral strategy for SARS-CoV-2 and treatment strategy for COVID-19 patients, although further studies are required to determine the mechanism of inhibition and potential efficacy in patients.
Institute
University of California, Los Angeles
DepartmentBiomedical Sciences
LaboratoryHeather Christofk
Last NameMatulionis
First NameNedas
Address615 Charles E Young Dr S, BSRB 354-05
Emailnmatulionis@mednet.ucla.edu
Phone310-206-0163
Submit Date2021-02-19
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2021-02-24
Release Version1
Nedas Matulionis Nedas Matulionis
https://dx.doi.org/10.21228/M89394
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Cultured cells; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Cell_type Sample_type
SA159041Sample_08HEK293T_ACE2 Mock
SA159042Sample_09HEK293T_ACE2 Mock
SA159043Sample_07HEK293T_ACE2 Mock
SA159044Sample_02HEK293T_ACE2 Mock
SA159045Sample_03HEK293T_ACE2 Mock
SA159046Sample_01HEK293T_ACE2 Mock
SA159047Sample_10HEK293T_ACE2 SARS-CoV-2
SA159048Sample_11HEK293T_ACE2 SARS-CoV-2
SA159049Sample_12HEK293T_ACE2 SARS-CoV-2
SA159050Sample_04HEK293T_ACE2 SARS-CoV-2
SA159051Sample_06HEK293T_ACE2 SARS-CoV-2
SA159052Sample_05HEK293T_ACE2 SARS-CoV-2
SA159053Sample_25NHBE-ALI Mock
SA159054Sample_26NHBE-ALI Mock
SA159055Sample_27NHBE-ALI Mock
SA159056Sample_29NHBE-ALI SARS-CoV-2
SA159057Sample_28NHBE-ALI SARS-CoV-2
SA159058Sample_30NHBE-ALI SARS-CoV-2
SA159059Sample_33Vero Mock
SA159060Sample_31Vero Mock
SA159061Sample_39Vero Mock
SA159062Sample_32Vero Mock
SA159063Sample_15Vero Mock
SA159064Sample_14Vero Mock
SA159065Sample_13Vero Mock
SA159066Sample_37Vero Mock
SA159067Sample_38Vero Mock
SA159068Sample_20Vero Mock
SA159069Sample_19Vero Mock
SA159070Sample_21Vero Mock
SA159071Sample_41Vero SARS-CoV-2
SA159072Sample_42Vero SARS-CoV-2
SA159073Sample_36Vero SARS-CoV-2
SA159074Sample_40Vero SARS-CoV-2
SA159075Sample_22Vero SARS-CoV-2
SA159076Sample_17Vero SARS-CoV-2
SA159077Sample_16Vero SARS-CoV-2
SA159078Sample_18Vero SARS-CoV-2
SA159079Sample_23Vero SARS-CoV-2
SA159080Sample_34Vero SARS-CoV-2
SA159081Sample_24Vero SARS-CoV-2
SA159082Sample_35Vero SARS-CoV-2
Showing results 1 to 42 of 42
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