Summary of Study ST000268

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000187. The data can be accessed directly via it's Project DOI: 10.21228/M8388M This work is supported by NIH grant, U2C- DK119886.

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Study IDST000268
Study TitleActivity-Based Metabolic Profiling of M. tuberculosis H37Rv Rv3311
Study Typetimecourse
Study SummaryThese experiments involve incubating various proteins of unknown function in the M. tuberculosis genome with whole-cell lysate and assaying changes in metabolite levels over time (e.g. 0, 15, 30min) to try to infer what reaction they might catalyze.
Institute
Weill Cornell Medicine
DepartmentMicrobiology and Immunology
Last NameRhee
First NameKyu
AddressDepartment of Microbiology and Immunology, Weill Cornell Medical College, 1300 York Avenue, New York, NY
Emailkyr9001@med.cornell.edu
Submit Date2015-11-13
Num Groups3 replicates X 3 timepoints
Study CommentsThe timepoints in the datafiles refer the amount of time (in minutes) that each protein was incubated with lysate.The units for 'mzmed' are mass/charge.
The units for 'rtmed' are seconds (retention time).
The units for all the other data columns in the spreadsheets are arbitrary; they are integrated peak counts.
In this study, Rv1713 was incubated with lysate with and without 1mM GTP added as a co-factor.
Raw Data AvailableNo
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2016-12-22
Release Version2
Release CommentsUpdated study design factors
Kyu Rhee Kyu Rhee
https://dx.doi.org/10.21228/M8388M
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Bacterial cells; Subject species: Mycobacterium tuberculosis H37Rv (Factor headings shown in green)

mb_sample_id local_sample_id timepoint (min)
SA012075Rv3311_A_00
SA012078Rv3311_B_00
SA012081Rv3311_C_00
SA012076Rv3311_A_1515
SA012079Rv3311_B_1515
SA012082Rv3311_C_1515
SA012077Rv3311_A_3030
SA012080Rv3311_B_3030
SA012083Rv3311_C_3030
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