Summary of Study ST003520
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002165. The data can be accessed directly via it's Project DOI: 10.21228/M8S247 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST003520 |
| Study Title | Identification of Plasma Metabolomic Biomarkers of Juvenile Idiopathic Arthritis |
| Study Type | Clinical |
| Study Summary | This study utilizes plasma metabolomic profiling to identify biomarkers associated with juvenile idiopathic arthritis (JIA) by analyzing samples from treatment-naïve JIA patients and non-JIA controls. Significant metabolic alterations were detected, with sphingosine metabolites and fatty acid ethanolamides showing notable increases in JIA patients, while specific compounds such as sarcosine were decreased. The research highlights 11 highly discriminatory metabolites, including sphinganine-1-phosphate, demonstrating potential for improved JIA diagnosis and treatment through targeted metabolic profiling. |
| Institute | University of Kansas |
| Department | Center for Computational Biology |
| Laboratory | Funk |
| Last Name | Kumar |
| First Name | Amar |
| Address | Multidisciplinary Research Bldg. 2030 Becker Drive Lawrence, KS 66047 |
| amarkumar@ku.edu | |
| Phone | 18723016225 |
| Submit Date | 2024-09-09 |
| Num Groups | 2 |
| Total Subjects | 210 |
| Num Males | 82 |
| Num Females | 128 |
| Study Comments | Although the raw dataset initially included 210 subjects, only 207 were included in the final analysis due to consent-related exclusions. These three subjects were removed to ensure compliance with ethical standards. |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-11-01 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO003642 |
| Collection Summary: | EDTA (Ethylenediaminetetraacetic acid) plasma specimens were systematically harvested from distinct patient cohorts under uniform preservative protocols and collection methodologies, although executed across disparate facilities by varying technical staff. The bio-samples involved two primary groups: patients diagnosed with Juvenile Idiopathic Arthritis (JIA) prior to the initiation of disease-modifying antirheumatic drugs (DMARDs), and a reference non-JIA pediatric population including children with active Crohn’s disease and healthy controls without autoimmune or discernible gastrointestinal or rheumatologic pathology. For JIA, plasma samples were procured in two phases: the discovery cohort consisting of 60 subjects from Children’s Mercy Kansas City (CM-KC), and the replication cohort comprising 49 subjects enrolled through PROMOTE studies at both CM-KC and Cincinnati Children’s Hospital Medical Center (CCHMC), Ohio. The non-JIA samples (n = 98) were sourced from a biorepository at CM-KC, collected in a fasted state during morning hours (7:30–11:30 A.M.), and were all from subjects who were age-matched to the JIA group and showed no organic causes for gastrointestinal symptoms, including no significant findings on histopathology from tissues biopsied during endoscopy. All specimens at CM-KC were gathered from subjects who had fasted for at least 8 hours, ensuring consistency in sample conditions. Patients provided age-appropriate informed consent or assent, and all sample collections were conducted under IRB-approved protocols to uphold ethical standards in research. Upon receiving, the venous blood samples were centrifuged using a Beckman tabletop centrifuge at 2000 RPM for 10 minutes to separate plasma. The resultant plasma supernatant was then aliquoted and preserved at -80°C. Prior to undergoing global metabolomic analysis, these samples were shipped on dry ice. Please note that for the analysis, both the datasets were combined into one dataset i.e., merged dataset, for more information please refer to the manuscript. DOI : https://doi.org/10.3390/metabo14090499 |
| Sample Type: | Blood (plasma) |
| Storage Conditions: | -80℃ |
