Summary of Study ST003049
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001899. The data can be accessed directly via it's Project DOI: 10.21228/M88147 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003049 |
| Study Title | Plasma instead of serum avoids critical confounding of clinical metabolomics studies by platelets (Part 2/3 - Eicosadomics of isolated platelets) |
| Study Summary | Metabolomics is an emerging and powerful molecular profiling method supporting clinical investigations. Serum and plasma are commonly used without rational prioritization. Serum is collected after blood coagulation, a complex biochemical process involving active platelet metabolism. This may affect the metabolome and increase the variance as platelet counts and function may vary substantially in individuals. A multi-omics approach systematically investigating the suitability of serum and plasma for clinical studies demonstrated that metabolites correlated well (n=461, R2=0.991), whereas lipid mediators (n=104, R2=0.906) and proteins (n=322, R2=0.860) differed substantially between specimen. Independently, analysis of platelet releasates identified most biomolecules significantly enriched in serum when compared to plasma. A prospective, randomized, controlled parallel group metabolomics trial with acetylsalicylic acid administered for 7 days demonstrated that the apparent drug effects significantly differ depending on analyzed specimen. Only serum analyses of healthy individuals suggested a significant downregulation of TXB2 and 12-HETE, which were specifically formed during coagulation in vitro. Plasma analyses reliably identified acetylsalicylic acid effects on metabolites and lipids occurring in vivo such as a decrease in polyunsaturated fatty acids. The present data suggests that plasma should be preferred above serum for clinical metabolomics studies as the serum metabolome may be substantially confounded by platelets. |
| Institute | University of Vienna |
| Department | Department of Analytical Chemistry |
| Laboratory | Gerner lab |
| Last Name | Hagn |
| First Name | Gerhard |
| Address | Währingerstraße 38, 1090 Vienna, Austria |
| gerhard.hagn@univie.ac.at | |
| Phone | +43 1 4277 52375 |
| Submit Date | 2024-01-22 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-04-12 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO003157 |
| Collection Summary: | PLATELETS: Whole blood of six healthy donors (three male and three female) in the age range of 26 to 51 years were collected in biological duplicates with one week in between the donations. Each donor gave written consent and the study was approved by the ethics committee of the Medical University of Vienna in accordance with the Declaration of Helsinki (EC 1430/2018). No medical substances interfering with the normal physiology of platelets such as aspirin, paracetamol or ibuprofen were taken by the donors 48 hours prior to blood donation. Two CPDA (citrate-phosphate-dextrose-adenine)-S-Monovette tubes (Sarstedt) of venous blood were collected per donor and donation. To isolate platelet rich plasma (PRP), the tubes were centrifuged for 20 min at 100 g with acceleration and deceleration set to 4. To purify platelets, size exclusion chromatography using 2 % B agarose beads (50-150 μm; abtbeads.es) was performed. Therefore, columns were equipped with a cotton frit and 20 mL of reconstituted agarose bead solution diluted 1:2 in RPMI medium (1X with L-Glutamine; Gibco, Thermo Fischer Scientific, Austria). Columns were washed with 2 mL RPMI medium before 1 ml of PRP was carefully pipetted to the column and topped with RPMI. The fraction containing purified platelets was collected and divided in two aliquots, one for platelet activation and one serving as control. To achieve platelet activation, ionomycin calcium salt (Sigma-Aldrich) was added to one aliquot to a final concentration of 1 μM. All samples were incubated for 15 min at room temperature before centrifugation at 2000 g for 5 min. |
| Sample Type: | Blood (isolated platelets) |
