Summary of Study ST002839

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001778. The data can be accessed directly via it's Project DOI: 10.21228/M8WB1S This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002839
Study TitleMetabolic alteration during ferroptotic process in cancer cells.
Study SummaryTargeting ferroptosis, an iron-dependent form of regulated cell death triggered by the lethal overload of lipid peroxides, in cancer therapy is impeded by our limited understanding of the intersection of tumour’s metabolic feature and ferroptosis vulnerability. In this study, we performed metabolomics in cancer cell lines pretreated with ferroptosis inducer RSL3 and control DMSO. We noted that the levels of a series of metabolites were significantly impacted by the RSL3 treatment, such as upregulated polyamines including putrescine, spermidine, and spermine. According to our previous study, we proved the pro-ferroptotic feature of polyamines in tumor cells, which was derived from the H2O2 produced during the polyamine metabolic process. This finding is consistent with our RNA-Seq data indicating upregulated ODC1 in the ferroptotic process. Therefore, it could be speculated that the RSL3-induced polyamine production leads to a positive-feedback loop that amplifies ferroptosis in tumor cells.
Zhongshan Hospital Fudan University
Last NameBi
First NameGuoshu
AddressZhongshan Hospital, Fudan University, No. 180, Fenglin Road, Xuhui District, Shanghai, China
Phone86 64041990
Submit Date2023-08-25
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2023-09-07
Release Version1
Guoshu Bi Guoshu Bi application/zip

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Collection ID:CO002942
Collection Summary:For cell samples, after being washed with ice-cold PBS, the cells were collected using a cell scraper and centrifuged for 5 min at 1,000 g. The samples were homogenized in liquid N2 for 1 min and defrosted in 4°C.
Sample Type:Tumor cells