Summary of Study ST002789

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001738. The data can be accessed directly via it's Project DOI: 10.21228/M82719 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002789
Study TitleMetabolomic analysis of particulate matter in the NPSG during a 2017 cruise on the R/V Kilo Moana as part of the MESOSCOPE program
Study SummaryTargeted and untargeted analysis of metabolomics samples from the North Pacific Subtropical Gyre taken during the 2017 June/July SCOPE cruise on the R/V Kilo Moana (KM1709) across a mesoscale eddy dipole, with high-resolution depth profile sampling across the DCM in the center of each eddy. Particulate matter was collected on 0.2um filters and extracted using a modified Bligh & Dyer before analysis on a QE Orbitrap. Results show significant changes in the absolute quantity and relative composition of particles in the gyre between anticyclonic and cyclonic eddies.
Institute
University of Washington
DepartmentSchool of Oceanography
LaboratoryIngalls Lab
Last NameKumler
First NameWilliam
Address1501 NE Boat St, Seattle, WA 98105
Emailwkumler@uw.edu
Phone2062216732
Submit Date2023-07-19
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2023-08-10
Release Version1
William Kumler William Kumler
https://dx.doi.org/10.21228/M82719
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Collection:

Collection ID:CO002889
Collection Summary:Samples were collected aboard the R/V Kilo Moana in June and July 2017. Water was collected with Niskin bottles attached to the CTD from the surface and the deep chlorophyll maximum (DCM), and 175 meters depth. Water was filtered onto a 0.2um pore size filter under gentle vacuum pressure prior to flash-freezing in LN2 before storage at -80 in precombusted aluminum foil. Samples were collected in two stages, the first of which was a transect across the entire eddy dipole from one edge to another while the second stage was a long-term Lagrangian occupation of the eddy centers (L1 = cyclone @ -12cm, L2 = anticyclone @ +25cm). Samples for the transect were collected throughout the day while the eddy center samples were all collected at about 6pm.
Sample Type:Suspended Marine Particulate Matter
Collection Method:CTD Niskin Bottle
Collection Location:North Pacific
Volumeoramount Collected:10L
Storage Conditions:-80℃
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