Summary of Study ST002270

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001452. The data can be accessed directly via it's Project DOI: 10.21228/M81Q5B This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002270
Study TitleXenopus tropicalis glycolysis and PPP inhibition
Study SummaryStage 41 tadpoles were injected with 4 nmol of the glycolysis inhibitor, 2-deoxyglucose (2DG), or a tracer control to evaluate consequences of inhibition on metabolites 24 hours after treatment began. Similarly, tadpoles were incubated in DMSO control or 1 of 2 G6PD inhibitors (Dehydroepiandrosterone and g6pdi), to similarly assess the consequences of inhibiting the pentose phosphate pathway. We find that inhibition of glucose metabolism with 2DG results in a decrease in downstream glycolytic intermediates, confirming a reduction in activity of this pathway. G6PD inhibition was not as clear as changes were less consistent across treatments and downstream metabolites did not behave in a coordinated way, though impacts on other metabolic processes by these inhibitors may be fruitful for exploring how they perturb metabolism in the tadpoles.
University of Washington
Last NamePatel
First NameJeet
Address1705 NE Pacific St., HSB J-Wing, J405, Seattle, Washington, 98195, USA
Submit Date2022-08-03
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2022-10-25
Release Version1
Jeet Patel Jeet Patel application/zip

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Collection ID:CO002349
Collection Summary:Tadpoles were anesthetized with 0.05% MS-222 in 1/9x MR and tested for response to touch prior to tissue collection. Injections of miniRuby or 4nmol of 2DG were performed or tadpoles were incubated in media with DMSO, DHEA, or g6pdi/ 10 whole animals were collected at 24 hours post treatment, media was removed, and samples were frozen on dry ice immediately.
Sample Type:Stage 43 tadpoles