Summary of Study ST000955
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000656. The data can be accessed directly via it's Project DOI: 10.21228/M8W39D This work is supported by NIH grant, U2C- DK119886. See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000955 |
| Study Title | Effects of ODC inhibition on T cell metabolism |
| Study Type | drug treatment at two time |
| Study Summary | Isolated T cells were activated for 48-72 hours in the presence and absence of 5mM DFMO, an ODC inhibitor |
| Institute | University of Florida |
| Department | SECIM |
| Last Name | Hesterberg |
| First Name | Rebecca |
| Address | 12902 Magnolia Dr |
| rebecca.hesterberg@moffitt.org | |
| Phone | 813-270-9181 |
| Submit Date | 2018-04-14 |
| Num Groups | 4 |
| Total Subjects | 16 |
| Study Comments | OT-1 mice express a transgenic T cell receptor in all CD8+ T cells that binds with high affinity to a peptide derived from the protein ovalbumin (SIINFEKL). |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2019-05-15 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO000988 |
| Collection Summary: | At 48 or 72 hours cells in culture medium were centrifuged on a Ficoll gradient to enrich viable cells. Cells were washed 1x with PBS, counted, and frozen as cell pellets at -80. |
| Sample Type: | Cultured cells |