Summary of Study ST000465
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000357. The data can be accessed directly via it's Project DOI: 10.21228/M8WW32 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000465 |
Study Title | Uniquely Tumor-Selective Englerin A Profoundly Alters Lipid Metabolism in Renal Cell Carcinoma inducing ER-Stress and an Acute Inflammatory Response |
Study Type | Metabolomic effect of Englerin A on renal cell carcinoma |
Study Summary | This targeted metabolomic analysis was performed on renal cell carcinoma A498 cells with or without anti-cancer drug Englerin treatment for 24 and 48 h. |
Institute | University of California, San Diego |
Department | Department of Pediatrics |
Last Name | Batova |
First Name | Ayse |
Address | La Jolla, CA 92093 |
abatova@ucsd.edu | |
Phone | 619-543-1962 |
Submit Date | 2016-09-09 |
Num Groups | Two groups for 24 h treatment (control and Eglerin treatment) and two groups for 48 h treatment. Each has 4 replicates |
Total Subjects | 16 |
Analysis Type Detail | LC-MS |
Release Date | 2016-12-22 |
Release Version | 1 |
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Collection:
Collection ID: | CO000480 |
Collection Summary: | Cell extracts were obtained by the addition of 1 ml of ice-cold methanol:water (80:20) to each dish followed by scraping cells into 1.7ml Eppendorf tubes and vortexing vigorously for 30 sec. A 50 ul aliquot of sample was removed for the picogreen DNA assay for purposes of biomass normalization, and the remaining sample was kept at -80˚C until ready for metabolomic analysis. |
Sample Type: | Kidney |