Summary of Study ST004274
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002700. The data can be accessed directly via it's Project DOI: 10.21228/M8NK11 This work is supported by NIH grant, U2C- DK119886. See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST004274 |
| Study Title | Loss of vitamin C biosynthesis protects from the pathology of a Schistosome infection and egg production. |
| Study Type | Treatment experiment |
| Study Summary | The processes influenced by ascorbate treatment in schistosome reproduction were investigated by metabolomics analysis of Ascorbate-treated and -untreated worm samples in the culture media. The top metabolite enriched in ascorbate-treated schistosomes was L-DOPA, a product of tyrosinase activity, which crosslinks vitellocyte proteins to form the schistosome eggshell, consistent with a role for ascorbate in vitellocyte development. |
| Institute | University of Texas Southwestern Medical Center at Dallas |
| Department | Children’s Medical Center Research Institute |
| Laboratory | Michalis Agathokleous |
| Last Name | Agathokleous |
| First Name | Michalis |
| Address | 6000 Harry Hines Blvd, NL12.110N, Dallas, TX, 75235 |
| michail.agathokleous@utsouthwestern.edu | |
| Phone | 2146486270 |
| Submit Date | 2025-10-06 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2025-10-31 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN007113 |
|---|---|
| Chromatography ID | CH005404 |
| MS ID | MS006810 |
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Thermo Scientific QExactive HF-X hybrid quadrupole orbitrap high-resolution mass spectrometer (HRMS) |
| Column | Millipore ZIC-pHILIC column (150 x 2.1 mm, 5 μm) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive HF-X Orbitrap |
| Ion Mode | UNSPECIFIED |
| Units | area under curve |
Chromatography:
| Chromatography ID: | CH005404 |
| Instrument Name: | Thermo Scientific QExactive HF-X hybrid quadrupole orbitrap high-resolution mass spectrometer (HRMS) |
| Column Name: | Millipore ZIC-pHILIC column (150 x 2.1 mm, 5 μm) |
| Column Temperature: | 40 |
| Flow Gradient: | 0–15 min linear ramp from 90% B to 30% B; 15–18 min isocratic flow of 30% B; 18–19 min linear ramp from 30% B to 90% B; 19–27 column regeneration with isocratic flow of 90% B. |
| Flow Rate: | 0.25 mL/min |
| Solvent A: | 100% water; 10 mM ammonium acetate, pH 9.8 |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | HILIC |
