Summary of Study ST003702
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002234. The data can be accessed directly via it's Project DOI: 10.21228/M8VJ9K This work is supported by NIH grant, U2C- DK119886. See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST003702 |
| Study Title | Kupffer cells control neonatal hepatic glucose metabolism via Igf1 signaling - lipidomics analysis of postnatal day 0 murine livers after macrophages depletion using Csf1r conditional KO |
| Study Type | lipidomics analysis of P0 livers from mice after macrophages depletion by Csf1r conditional KO |
| Study Summary | During perinatal development, liver metabolism is tightly regulated to ensure energy supply for the newborn. Before birth, glycogen is stored in hepatocytes and later metabolized to glucose, meeting the energy demands of the neonate. Shortly after birth, lipogenesis begins, driven by the transcriptional activation of enzymes involved in fatty acid oxidation. These processes are thought to be largely regulated by systemic insulin and glucagon levels. However, the role of liver-derived local factors in neonatal hepatocyte metabolism remains unexplored. Kupffer cells (KCs), the liver’s resident macrophages, colonize the fetal liver early in embryogenesis and support liver metabolism in adulthood. Yet, whether KCs influence neonatal hepatocyte metabolism is unknown. Here, using conditional knockout mouse models targeting macrophages (Csf1r-flox Tnfrsf11a-Cre), we demonstrate that yolk sac-derived KCs play a critical role in hepatocyte glycogen storage and function by regulating the TCA cycle - a role that monocyte-derived KC-like cells cannot substitute. in order to check the lipid levels after birth, Newborn pups were collected and the different lipid species were assessd by lipidomics analysis using mass spectrometer. |
| Institute | University of Bonn |
| Department | Developmental Biology of the Immune System, The Life & Medical Sciences Institute (LIMES) |
| Laboratory | Mass Lab |
| Last Name | Makdissi |
| First Name | Nikola |
| Address | Carl Troll straße 31 |
| nmakdissi@uni-bonn.de | |
| Phone | 02 28 / 73 - 6 2794 |
| Submit Date | 2025-01-15 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2025-02-11 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN006074 |
|---|---|
| Chromatography ID | CH004613 |
| MS ID | MS005781 |
| Analysis type | MS |
| Chromatography type | None (Direct infusion) |
| Chromatography system | none |
| Column | none |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Plus Orbitrap |
| Ion Mode | POSITIVE |
| Units | pmol/g liver |
Chromatography:
| Chromatography ID: | CH004613 |
| Instrument Name: | none |
| Column Name: | none |
| Column Temperature: | none |
| Flow Gradient: | none |
| Flow Rate: | none |
| Solvent A: | none |
| Solvent B: | none |
| Chromatography Type: | None (Direct infusion) |
