Summary of Study ST003672
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002277. The data can be accessed directly via it's Project DOI: 10.21228/M89G0H This work is supported by NIH grant, U2C- DK119886. See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST003672 |
| Study Title | Advanced Lipidomics Using UHPLC-ESI-QTOF-MS/MS Reveals Novel Lipids in Hibernating Syrian Hamsters |
| Study Summary | Mammalian hibernation offers a unique model for exploring neuroprotective mechanisms relevant to neurodegenerative diseases. In this study, we employed untargeted lipidomics with iterative tandem mass spectrometry (MS/MS) to profile the brain lipidome of Syrian hamsters across different hibernation stages: late torpor, arousal, and euthermia (control). Previously, a lipid species identified as methyl-PA(16:0/0:0) showed a significant increase during torpor, but its precise structure was unresolved due to technological constraints. Leveraging iterative MS/MS and advanced lipid annotation tools (LipidAnnotator and MS-DIAL), we accurately annotated 377 lipid species, including the re-identification of methyl-PA(16:0/0:0) as methylated lysophosphatidic acid (PMeOH 16:0/0:0). This reannotation led to the discovery of two additional lipids during torpor: PMeOH 18:0/0:0 and PMeOH 18:1/0:0. Verification involved manual inspection of MS/MS spectra and Kendrick Mass Defect plots. The lipid alterations observed during torpor suggest biochemical adaptations to maintain membrane fluidity and protect against oxidative stress under hypothermic conditions. Elevated levels of PMeOH lipids and their lyso-forms may play roles in cell survival signalling. Additionally, a decrease in phosphatidic acid species and an increase in diacylglycerol species imply a metabolic shift favouring diacylglycerol production, potentially activating protein kinase C signalling pathways. The increased levels of monogalactosyl diglyceride lipids during torpor suggest a role in neuroprotection by enhancing oligodendrocyte function and myelination. Our comprehensive lipidomic profiling provides detailed insights into lipid dynamics associated with hibernation and underscores the potential of advanced MS/MS methodologies in lipidomics for developing therapeutic strategies against neurodegenerative diseases. |
| Institute | Universidad CEU San Pablo |
| Department | Centro de Metabolómica y Bioanálisis (CEMBIO) |
| Last Name | González |
| First Name | Carolina |
| Address | km 0, Universidad CEU-San Pablo Urbanización Montepríncipe. M-501 |
| carolina.gonzalezriano@ceu.es | |
| Phone | 646251045 |
| Submit Date | 2025-01-17 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML |
| Analysis Type Detail | LC-MS |
| Release Date | 2025-02-05 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN006029 | AN006030 |
|---|---|---|
| Chromatography ID | CH004582 | CH004582 |
| MS ID | MS005740 | MS005741 |
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Agilent 1290 Infinity II | Agilent 1290 Infinity II |
| Column | Agilent InfinityLab Poroshell 120 EC-C18 (100 x 3mm,2.7um) | Agilent InfinityLab Poroshell 120 EC-C18 (100 x 3mm,2.7um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Agilent 6545 QTOF | Agilent 6545 QTOF |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak area | Peak area |
Chromatography:
| Chromatography ID: | CH004582 |
| Chromatography Summary: | The Agilent 1290 Infinity II Multisampler system was employed with a multiwash option, and 1 µL of extracted samples was injected. The multisampler temperature was maintained at 15 °C to ensure the stability of compounds and prevent lipid precipitation. For chromatographic separation, an Agilent InfinityLab Poroshell 120 ECC18 (3.0 × 100 mm, 2.7 µm) (Agilent Technologies) column and a compatible guard column (Agilent InfinityLab Poroshell 120 ECC18, 3.0 × 5 mm, 2.7 µm) were employed and held at 50 °C. The chromatography gradient was initiated at 70% of B at 0–1 min, increased to 86% at 3.5–10 min, and reached 100% B at 11–17 min. The initial conditions were restored by minute 17, followed by a 2-minute re-equilibration, resulting in a total running time of 19 min. The mobile phases for positive and negative ionization modes comprised (A) 10 mM ammonium acetate, 0.2 mM ammonium fluoride in a 9:1 water/methanol ratio and (B) 10 mM ammonium acetate, 0.2 mM ammonium fluoride in a 2:3:5 acetonitrile/methanol/isopropanol ratio. The flow rate was maintained at 0.6 mL/min. The multisampler's multiwash strategy involved a methanol:isopropanol (50:50, v/v) mixture with a 15-second wash time and an aqueous:organic phases (30:70, v/v) mixture to achieve the initial conditions. |
| Instrument Name: | Agilent 1290 Infinity II |
| Column Name: | Agilent InfinityLab Poroshell 120 EC-C18 (100 x 3mm,2.7um) |
| Column Temperature: | 50 °C |
| Flow Gradient: | Started at 70% of B at 0 –1 min, 86% B at 3.5 –10 min, and 100% B at 11–17 min |
| Flow Rate: | 0.6 mL/min |
| Solvent A: | 90% water/10% methanol; 10 mM ammonium acetate; 0.2 mM ammonium fluoride |
| Solvent B: | 20% acetonitrile/30% methanol/50% isopropanol; 10 mM ammonium acetate; 0.2 mM ammonium fluoride |
| Chromatography Type: | Reversed phase |
