Summary of Study ST003527
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002170. The data can be accessed directly via it's Project DOI: 10.21228/M84C19 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003527 |
| Study Title | Combining antibiotics alters the longitudinal maturation of gut microbiota and its short chain fatty acid metabolites in extremely and very preterm infants |
| Study Summary | Antibiotics are routinely prescribed to extremely and very premature infants as a pre-emptive and prophylactic treatment to reduce the risk of acute neonatal illness (i.e. necrotizing enterocolitis, NEC) associated with morbidity. To investigate the effects of antibiotic types, combinations, and duration on the preterm gut microbiome and metabolome, we analyzed the microbiome compositions of 123 stool samples collected at 3 timepoints (postnatal day 1, 28 and 56) from extremely- and very-low-birthweight infants treated with 14 different antibiotics spanning across 5 classes. Targeted metabolomics were performed on 47 samples available, allowing us to quantify 649 metabolites including amino acids, bile acids, fatty acids, and lipids. As a result, we found that antibiotics exerted the most profound disruptive impact on the gut microbiota, while antibiotics and breastfeeding highly influence the gut metabolome. Short chain fatty acids were reduced in both antibiotic-treated and NEC group. Finally, we revealed that cephalosporins negatively impact conjugated bile acids due to a positive correlation with bile salt hydrolase-producing Staphylococcus. |
| Institute | Seoul National University |
| Last Name | Kyeong-Seog |
| First Name | Kim |
| Address | Jongno-Gu, South Korea |
| 92kkim@gmail.com | |
| Phone | +8227408905 |
| Submit Date | 2024-09-23 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d, wiff |
| Analysis Type Detail | GC-MS/LC-MS |
| Release Date | 2024-10-22 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN005792 | AN005793 | AN005794 |
|---|---|---|---|
| Chromatography ID | CH004397 | CH004398 | CH004399 |
| MS ID | MS005512 | MS005513 | MS005514 |
| Analysis type | MS | MS | MS |
| Chromatography type | GC | Reversed phase | None (Direct infusion) |
| Chromatography system | Agilent 7890B | Waters Acquity | Waters Acquity |
| Column | Agilent DB-FFAP (30m × 0.25mm, 0.25um) | Biocrates MxP Quant 500 (XL) PN 21117 | NA (FIA mode) |
| MS Type | EI | ESI | ESI |
| MS instrument type | Triple quadrupole | Triple quadrupole | Triple quadrupole |
| MS instrument name | Agilent 7000B | ABI Sciex Triple Quad 5500+ | ABI Sciex Triple Quad 5500+ |
| Ion Mode | POSITIVE | UNSPECIFIED | UNSPECIFIED |
| Units | uM | uM | uM |
Chromatography:
| Chromatography ID: | CH004397 |
| Chromatography Summary: | Selected Ion Monitoring (SIM) was applied to measure the short-chain fatty acid in feces. The m/z values at 60 (acetic acid, butyric acid, and isovaleric acid), 73 (isobutyric acid), and 74 (propionic acid) was applied. |
| Instrument Name: | Agilent 7890B |
| Column Name: | Agilent DB-FFAP (30m × 0.25mm, 0.25um) |
| Column Temperature: | an initial GC oven temperature was 40°C, held for 2 min, increased by 40°C/min to 200°C. The post-run time was 6 min at 240°C |
| Flow Gradient: | NA |
| Flow Rate: | NA |
| Solvent A: | NA |
| Solvent B: | NA |
| Chromatography Type: | GC |
| Chromatography ID: | CH004398 |
| Instrument Name: | Waters Acquity |
| Column Name: | Biocrates MxP Quant 500 (XL) PN 21117 |
| Column Temperature: | 50 |
| Flow Gradient: | Flow Gradient LC1: 0% B (0.25 min) -> 12% B (1.25 min) -> 17.5% B (1.2 min) -> 50% B (1.3 min) -> 100% B (0.5 min) -> stay at 100% B (0.5 min) -> 0% B (0.1 min) -> held at 0% B (0.7 min), total 5.8 min; Flow Gradient LC2: 0% B (0.25 min) -> 25% B (0.25 min) -> 50% B (1.5 min) -> 75% B (1.0 min) -> 100% B (0.5 min) -> stay at 100% B (1.5 min) -> 0% B (0.1 min) -> held at 0% B (0.7 min), total 5.8 min |
| Flow Rate: | 0.8 mL/min, increased to 1.0 mL/min from minute 4.7 to 5.1 |
| Solvent A: | 100% water; 0.2% formic acid |
| Solvent B: | 95% acetonitrile/5% water; 0.2% formic acid |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH004399 |
| Instrument Name: | Waters Acquity |
| Column Name: | NA (FIA mode) |
| Column Temperature: | NA |
| Flow Gradient: | 100% B, 0-3min |
| Flow Rate: | 0.03mL/min (0-1.6min), 0.2mL/min (1.6-2.8min), Back to 0.03mL/min (2.8-3.0min) |
| Solvent A: | 100% water; 0.2% formic acid |
| Solvent B: | 95% acetonitrile/5% water; 0.2% formic acid |
| Chromatography Type: | None (Direct infusion) |
