Summary of Study ST002826
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001768. The data can be accessed directly via it's Project DOI: 10.21228/M85T5M This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002826 |
| Study Title | Cold-stimulated brown adipose tissue activation is related to changes in serum metabolites relevant to NAD+ metabolism in humans |
| Study Summary | Cold-induced brown adipose tissue (BAT) activation is considered to improve metabolic health. In murine BAT, cold increases the fundamental molecule for mitochondrial function, nicotinamide adenine dinucleotide (NAD+), but limited knowledge on NAD+ metabolism during cold in human BAT metabolism exists. We show that cold increases the serum metabolites of the NAD+ salvage pathway (nicotinamide and 1-methylnicotinamide) in humans. Additionally, individuals with coldstimulated BAT activation have decreased levels of metabolites from the de novo NAD+ biosynthesis pathway (tryptophan, kynurenine). Serum nicotinamide correlates positively with cold-stimulated BAT activation, whereas tryptophan and kynurenine correlates negatively. Furthermore, the expression of genes involved in NAD+ biosynthesis in BAT are related to markers of metabolic health. Our data indicate that cold increases serum tryptophan conversion to nicotinamide to be further utilized by BAT. We conclude that NAD+ metabolism is activated upon cold in humans and probably regulated in a coordinated fashion by several tissues. |
| Institute | University of Turku |
| Last Name | Virtanen |
| First Name | Kirsi |
| Address | Turku PET Centre, Turku University Hospital, Turku, Finland |
| kirsi.virtanen@utu.fi | |
| Phone | +358-40-7626564 |
| Submit Date | 2023-08-10 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-09-11 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004610 | AN004611 | AN004612 | AN004613 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | HILIC | HILIC | Reversed phase | Reversed phase |
| Chromatography system | Agilent 1290 | Agilent 1290 | Agilent 1290 | Agilent 1290 |
| Column | Acquity UPLC BEH Amide column, 2.1 × 100 mm, 1.7 μm | Acquity UPLC BEH Amide column, 2.1 × 100 mm, 1.7 μm | Agilent Zorbax Eclipse XDB-C18 Rapid Resolution HD 1.8 μm, 2.1 x 100 mm | Agilent Zorbax Eclipse XDB-C18 Rapid Resolution HD 1.8 μm, 2.1 x 100 mm |
| MS Type | ESI | ESI | ESI | ESI |
| MS instrument type | QTOF | QTOF | QTOF | QTOF |
| MS instrument name | Agilent 6540 QTOF | Agilent 6540 QTOF | Agilent 6540 QTOF | Agilent 6540 QTOF |
| Ion Mode | POSITIVE | NEGATIVE | POSITIVE | NEGATIVE |
| Units | Area | Area | Area | Area |
Chromatography:
| Chromatography ID: | CH003468 |
| Instrument Name: | Agilent 1290 |
| Column Name: | Acquity UPLC BEH Amide column, 2.1 × 100 mm, 1.7 μm |
| Column Temperature: | 45 |
| Flow Gradient: | 0–2.5 min: 100% B, 2.5–10 min: 100% B → 0% B; 10–10.01 min: 0% B → 100% B; 10.01–12.5 min: 100% B. |
| Flow Rate: | 0.6 mL/min. |
| Solvent A: | 50% acetonitrile with 20 mM ammonium formate and 0.25% formic acid, pH 3 |
| Solvent B: | 90% acetonitrile with 20 mM ammonium formate, 0.25% formic acid, pH 3. |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH003469 |
| Instrument Name: | Agilent 1290 |
| Column Name: | Agilent Zorbax Eclipse XDB-C18 Rapid Resolution HD 1.8 μm, 2.1 x 100 mm |
| Column Temperature: | 50 |
| Flow Gradient: | 2 to 100% B in 10 min; 100% B from 10 min to 14.5 min; 100% to 2% B, from 14.51 min to 16.5 min |
| Flow Rate: | 0.4 mL/min. |
| Solvent A: | water with 0.1% (v/v) of formic acid |
| Solvent B: | methanol with 0.1% (v/v) of formic acid |
| Chromatography Type: | Reversed phase |
