Summary of Study ST002817
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001748. The data can be accessed directly via it's Project DOI: 10.21228/M8RQ7M This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002817 |
| Study Title | Investigation of metabolism in hypertrophic cardiomyopathy - Lipidomics |
| Study Summary | We propose the use of targeted metabolomics to define the metabolic and molecular pathways altered in 2 mouse models (R92W-TnT and R403Q-MHC) of hypertrophic cardiomyopathy (HCM) that span the spectrum of human disease (heart failure and sudden death), with the goal of identifying treatment targets. Parallel targeted metabolomics studies will be performed in heart tissue and plasma at rest and following inotrope stimulation. Since energy compromise is expected to be most marked when the heart is subject to increased workload, as is the case during high-intensity exercise or inotropic stimulation, we propose metabolomics studies in heart tissue and plasma, at rest and following inotrope stimulation. We anticipate that the results of these studies will allow us to move forward with further investigations into specific metabolites of interest as biomarkers, to be tested in HCM patients in future studies. |
| Institute | University of California, San Francisco |
| Department | Hypertrophic Cardiomyopathy Center of Excellence |
| Laboratory | UCSF Smith Cardiovascular Research Building |
| Last Name | Abraham |
| First Name | Maria Roselle |
| Address | 555 Mission Bay Boulevard South, San Francisco, CA 94158 |
| Roselle.Abraham@ucsf.edu | |
| Phone | 415-502-3911 |
| Submit Date | 2023-07-27 |
| Num Groups | 2 groups of 2 (i.e., mutant vs control of two mouse models of HCM (R402Q-MyHC, R92W-TnT) |
| Total Subjects | 32 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-09-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004584 | AN004585 | AN004586 |
|---|---|---|---|
| Analysis type | MS | MS | MS |
| Chromatography type | Reversed phase | Reversed phase | Reversed phase |
| Chromatography system | Agilent 1290 | Agilent 1290 | Waters Acquity |
| Column | Waters ACQUITY UPLC CSH C18 (100 x 1mm,1.7um) | Waters ACQUITY UPLC CSH C18 (100 x 1mm,1.7um) | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) |
| MS Type | ESI | ESI | ESI |
| MS instrument type | QTOF | QTOF | Orbitrap |
| MS instrument name | Agilent 6530 QTOF | Agilent 6550 QTOF | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE | POSITIVE |
| Units | peak heights | peak heights | raw area counts rescaled to set the median equal to 1 |
Chromatography:
| Chromatography ID: | CH003445 |
| Chromatography Summary: | - |
| Instrument Name: | Agilent 1290 |
| Column Name: | Waters ACQUITY UPLC CSH C18 (100 x 1mm,1.7um) |
| Column Temperature: | 65C |
| Flow Gradient: | 0 min 15% (B), 0–2 min 30% (B), 2–2.5 min 48% (B), 2.5–11 min 82% (B), 11–11.5 min 99% (B), 11.5– 12 min 99% (B), 12–12.1 min 15% (B), 12.1–15 min 15% (B) |
| Flow Rate: | 0.6 mL/min |
| Solvent A: | 60:40 v/v acetonitrile:water + 10 mM ammonium formate + 0.1% formic acid |
| Solvent B: | 90:10 v/v isopropanol:acetonitrile + 10 mM ammonium formate + 0.1% formic acid |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH003446 |
| Chromatography Summary: | Low pH polar (LC/MS Pos early) |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) |
| Column Temperature: | - |
| Flow Gradient: | - |
| Flow Rate: | - |
| Solvent A: | - |
| Solvent B: | - |
| Chromatography Type: | Reversed phase |
