Summary of Study ST002794

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001742. The data can be accessed directly via it's Project DOI: 10.21228/M8J71N This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002794
Study TitleMetabolomics Study on Plasma and Lung Tissue of Rats Exposed to Whole Thorax Irradiation.
Study SummaryAlthough some progress has been made in the study of radiation injury, there are still no effective prevention and treatment methods for severe acute radiation syndrome or sickness (ARS). Accordingly, a thorough understanding of biological characteristics associated with high-dose radiation is essential for revealing the mechanisms underlying the varied biological processes following high dose radiation and the development of novel potent radioprotective agents. In present study, plasma metabolic characteristics were investigated from patients of hematopoietic stem cell transplantation following high-dose TBI pretreatment utilizing gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS). The most potential panel of four metabolic markers for radiation injury was selected and the metabolic disorders involved were explored. The metabolic disorders implied the dysregulation of gut microflora, shift of energy supply from aerobic respiration to ketogenesis, protein synthesis and metabolism in response to TBI. Although similar metabolic alternation patterns exist between male and female following high-dose irradiation, specific changes are observed in either male or female patients. These findings provide valuable information for further selecting biomarkers, clues of pathogenic mechanisms involved in high-dose radiation exposure.
Soochow University
Last NameWang
First NameChang
AddressSuzhou, No. 199, Renai Road, Suzhou Industrial Park
Submit Date2023-07-18
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2023-08-10
Release Version1
Chang Wang Chang Wang application/zip

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Combined analysis:

Analysis ID AN004545 AN004546
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo TSQ Vantage HPLC-MS/MS Thermo TSQ Vantage HPLC-MS/MS
Column Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um) Waters ACQUITY UPLC BEH C8 (100 x 2.1mm,1.7um)
MS instrument type Triple quadrupole Triple quadrupole
MS instrument name Thermo TSQ Vantage Thermo TSQ Vantage
Units peak area peak area


Chromatography ID:CH003414
Chromatography Summary:negtive ion mode
Instrument Name:Thermo TSQ Vantage HPLC-MS/MS
Column Name:Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um)
Column Temperature:55
Flow Gradient:0~1 min, 2% B; 1-20 min, 2~100% B; 20~24min, 100% B; 24~24.5min, 100~2% B; 24.5~34 min, 2% B
Flow Rate:0.35 mL/min
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile; 0.1% formic acid
Chromatography Type:Reversed phase
Chromatography ID:CH003415
Chromatography Summary:positive ion mode
Instrument Name:Thermo TSQ Vantage HPLC-MS/MS
Column Name:Waters ACQUITY UPLC BEH C8 (100 x 2.1mm,1.7um)
Column Temperature:50
Flow Gradient:0~0.5min, 5% B; 0.5 ~24min, 5~100% B; 24-28 min, 40-100% B; 28~28.5min, 100~5% B; 28.5~48 min, 5% B
Flow Rate:0.35 mL/min
Solvent A:water(0.1% formic acid )
Solvent B:acetonitrile(0.1% formic acid )
Chromatography Type:Reversed phase