Summary of Study ST002459
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001586. The data can be accessed directly via it's Project DOI: 10.21228/M8PQ70 This work is supported by NIH grant, U2C- DK119886. See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST002459 |
| Study Title | Comparison of metabolite profiles from matched whole blood microsamplers, whole blood dried blood spots, and plasma. |
| Study Summary | Venous blood was collected from 54 adult female participants from the PRISM cohort. Whole blood and venous blood was aliquoted. Untargeted metabolomics was performed on whole blood collected on Mitra microsamplers (VAMS, 10 uL), whole blood dried blood spots (DBS, 5-mm punch), and 10 uL of plasma |
| Institute | Icahn School of Medicine at Mount Sinai |
| Last Name | Petrick |
| First Name | Lauren |
| Address | 1428 Madison Avenue, Atran Building |
| lauren.petrick@mssm.edu | |
| Phone | 9739976141 |
| Submit Date | 2023-01-22 |
| Total Subjects | 54 |
| Num Females | 54 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-01-22 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004011 | AN004012 | AN004013 |
|---|---|---|---|
| Chromatography ID | CH002962 | CH002963 | CH002964 |
| MS ID | MS003758 | MS003759 | MS003760 |
| Analysis type | MS | MS | MS |
| Chromatography type | Reversed phase | Reversed phase | HILIC |
| Chromatography system | Agilent 6550 | Agilent 6550 | Agilent 6550 |
| Column | Agilent Zorbax StableBond Aq (50 x 2.1mm,1.8um) | Agilent ZORBAX Eclipse Plus C18 (50 x 2.1mm,1.8um) | SeQuant ZIC-HILIC (100 x 2.1mm,3.5um) |
| MS Type | ESI | ESI | ESI |
| MS instrument type | QTOF | QTOF | QTOF |
| MS instrument name | Agilent 6550 QTOF | Agilent 6550 QTOF | Agilent 6545 QTOF |
| Ion Mode | POSITIVE | NEGATIVE | POSITIVE |
| Units | Ion abundance | Ion abundance | Ion abundance |
Chromatography:
| Chromatography ID: | CH002962 |
| Chromatography Summary: | Reverse-phase positive |
| Instrument Name: | Agilent 6550 |
| Column Name: | Agilent Zorbax StableBond Aq (50 x 2.1mm,1.8um) |
| Column Temperature: | 60 |
| Flow Gradient: | 2–98% B in 13 min, hold at 98% B for 6 min |
| Flow Rate: | 0.550 mL/min flow rate |
| Solvent A: | 0.2% acetic acid in water |
| Solvent B: | 0.2% acetic acid in methanol |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH002963 |
| Chromatography Summary: | Reverse-phase negative |
| Instrument Name: | Agilent 6550 |
| Column Name: | Agilent ZORBAX Eclipse Plus C18 (50 x 2.1mm,1.8um) |
| Column Temperature: | 50 |
| Flow Gradient: | 5% B for 2 min, increasing to 30% B in 2 min, and increasing from 30% to 98% B in 9.5 min with a 1.5 min hold |
| Flow Rate: | 0.400 mL/min flow rate |
| Solvent A: | 0.1% formic acid in water |
| Solvent B: | 0.1% formic acid in (90:10, v/v) 2-propanol:ACN |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH002964 |
| Chromatography Summary: | HILIC positive |
| Instrument Name: | Agilent 6550 |
| Column Name: | SeQuant ZIC-HILIC (100 x 2.1mm,3.5um) |
| Column Temperature: | 25 |
| Flow Gradient: | 95% B for 1.5 min, decreasing to 40% B in 10.5 min, followed by a 2 min hold, a decrease to 25% B in 0.2 min and a hold for 2.8 min at 25%B |
| Flow Rate: | 0.300 mL/min flow rate |
| Solvent A: | 0.1% formic acid in water |
| Solvent B: | 0.1% formic acid in acetonitrile |
| Chromatography Type: | HILIC |
