Summary of Study ST002322
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001488. The data can be accessed directly via it's Project DOI: 10.21228/M8CT5G This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST002322 |
| Study Title | Metabolomics study comparing SCAP KO and WT B cells |
| Study Type | Purified mouse B cells, stimulated ex vivo |
| Study Summary | Splenic B cells isolated from SCAP+/+ CD19-Cre (WT) and SCAPfl/fl CD19-Cre (KO) mice were stimulated with LPS or anti-CD40 for 24 and 48 hours. Cells were then analyzed by metabolomics. Metabolomics reveals global metabolic changes in SCAP deficient B cells. |
| Institute | Indiana University School of Medicine |
| Last Name | Luo |
| First Name | Wei |
| Address | 950 W Walnut Street - R2 E304 |
| wl47@iu.edu | |
| Phone | 3172748042 |
| Submit Date | 2022-10-19 |
| Analysis Type Detail | LC-MS |
| Release Date | 2022-11-18 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003789 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase/HILIC |
| Chromatography system | Waters ACQUITY ultra-performance liquid chromatography (UPLC) |
| Column | Waters UPLC BEH C18 (100 x 2.1mm,1.7um)/Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Exactive Plus Orbitrap |
| Ion Mode | UNSPECIFIED |
| Units | Normalized AUC |
Chromatography:
| Chromatography ID: | CH002802 |
| Methods Filename: | B_cell_MS_protocol.docx |
| Instrument Name: | Waters ACQUITY ultra-performance liquid chromatography (UPLC) |
| Column Name: | Waters UPLC BEH C18 (100 x 2.1mm,1.7um)/Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
| Chromatography Type: | Reversed phase/HILIC |
