Summary of Study ST002282

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001462. The data can be accessed directly via it's Project DOI: 10.21228/M8R997 This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002282
Study TitleDetection of Methyl jasmonate (MeJA) in Plant root VOCs
Study SummaryMethyl jasmonate (MeJA) is a well-known plant hormone known for plant defense and plant-plant signaling. However, most of the studies are focussed on its aboveground presence and functions. Here we report that MeJA is also released by plant roots in a volatile form. More importantly, it is shown in Arabidopsis growing in natural conditions in soil.
National University of Singapore
Last NameKulkarni
First NameOmkar
AddressDept of Biological Sciences,Metabolites Biology Lab,, Science drive 4,Block S1A #06-03
Submit Date2022-08-25
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2022-11-25
Release Version1
Omkar Kulkarni Omkar Kulkarni application/zip

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Combined analysis:

Analysis ID AN003727
Analysis type MS
Chromatography type GC
Chromatography system Agilent 7890B
Column Agilent DB-FFAP
MS Type EI
MS instrument type Single quadrupole
MS instrument name Agilent 5977B
Units ion intensity peak area


Chromatography ID:CH002760
Chromatography Summary:Sample preparation and injection were performed using the fully automated Gerstel MPS-2 autosampler and Gerstel MAESTRO software. Volatile compounds were adsorbed on a Tenax TA tube. A thermal desorption unit (TDU) was used to thermally desorb the volatiles in splitless mode at 230°C for 10 min. To ensure that the volatiles released from the TDU were quantitatively trapped, a cooled injection system-programmed temperature vaporizer (CIS-PTV) was used. The CIS was heated from 80°C to 230°C at a rate of 12°C/s with the split valve closed during sample injection into the GC inlet. Analyses of volatile compounds were performed on an Agilent 7890B GC coupled to a 5977B quadruple mass spectrometer. Separation of compounds was performed on a DB-FFAP column (60 m x 250 µm x 0.25 µm, Agilent Technologies, Middleburg, OI, USA). Helium was used as the carrier gas at a flow rate of 1.9 ml/min, and solvent vent mode was used. The inlet temperature was 250°C. The oven program was as follows: initial temperature of 50°C held for 1 min, increased to 230°C at the rate of 10°C/min and held for 20 min. The temperature of the ion source and transfer line was 250°C.
Instrument Name:Agilent 7890B
Column Name:Agilent DB-FFAP
Chromatography Type:GC