Summary of Study ST002269
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001451. The data can be accessed directly via it's Project DOI: 10.21228/M85H8N This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002269 |
| Study Title | Targeted plasma metabolomics in pediatric NAFLD patients |
| Study Summary | Several adult omics studies have been conducted to understand the pathophysiology of nonalcoholic fatty liver disease (NAFLD). However, the histological features of children are different from those of adults, and the onset and progression of pediatric NAFLD are not fully understood. In this study, we aimed to evaluate the metabolome profile and metabolic pathway changes associated with pediatric NAFLD to elucidate its pathophysiology. We analyzed the metabolic profiles of healthy control, lean NAFLD, overweight control and overweight NAFLD groups of children and adolescent participants (n = 165) by assessing plasma samples, and identified 18 NAFLD-specific metabolic features and metabolic changes in lipid, glutathione-related amino acid, and branched-chain amino acid metabolism by comparing control and NAFLD group in pediatric population with overweight. Metabolome changes in the plasma of pediatric patients with NAFLD are associated with the pathophysiology of the disease and can be utilized as a less-invasive approach to diagnose the disease. |
| Institute | Seoul National University College of Medicine and Hospital |
| Department | Department of Clinical Pharmacology and Therapeutics |
| Last Name | Chae |
| First Name | Woori |
| Address | 101 Daehak-ro, Jongno-gu, Seoul 03080, Republic of Korea |
| yunus@snu.ac.kr | |
| Phone | 82-2-740-8905 |
| Submit Date | 2022-08-07 |
| Num Groups | 4 |
| Total Subjects | 165 |
| Num Males | 122 |
| Num Females | 43 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS/MS(Dir. Inf.) |
| Release Date | 2023-08-07 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003706 | AN003707 | AN003708 | AN003709 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | Reversed phase | Reversed phase | None (Direct infusion) | None (Direct infusion) |
| Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
| Column | Biocrates AbsoluteIDQ p400 HR Kit UHPLC (PN 9120052121032) | Biocrates AbsoluteIDQ p400 HR Kit UHPLC (PN 9120052121032) | NA | NA |
| MS Type | ESI | ESI | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Plus Orbitrap | Thermo Q Exactive Plus Orbitrap | Thermo Q Exactive Plus Orbitrap | Thermo Q Exactive Plus Orbitrap |
| Ion Mode | POSITIVE | POSITIVE | POSITIVE | POSITIVE |
| Units | μM | μM | μM | μM |
Chromatography:
| Chromatography ID: | CH002745 |
| Chromatography Summary: | LC1 method for analysis of amino acids and biogenic amines For more details, please refer sections 4.6, 4.7, 5, 9, 10.1, and 10.2 of the method file attached. |
| Methods Filename: | UM-p400-HR-Thermo-3.pdf |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Biocrates AbsoluteIDQ p400 HR Kit UHPLC (PN 9120052121032) |
| Column Temperature: | 50 |
| Solvent A: | 100% water; 0.2% formic acid |
| Solvent B: | 100% acetonitrile; 0.2% formic acid |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH002746 |
| Chromatography Summary: | LC2 method for analysis of isoleucine For more details, please refer sections 4.6, 4.7, 5, 9, 10.1, and 10.2 of the method file attached. |
| Methods Filename: | UM-p400-HR-Thermo-3.pdf |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Biocrates AbsoluteIDQ p400 HR Kit UHPLC (PN 9120052121032) |
| Column Temperature: | 50 |
| Solvent A: | 100% water; 0.2% formic acid |
| Solvent B: | 100% acetonitrile; 0.2% formic acid |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH002747 |
| Chromatography Summary: | FIA1 method for analysis of acylcarnitines and lipids For more details, please refer sections 4.6, 4.7, 5, 9, 10.1, and 10.2 of the method file attached. |
| Methods Filename: | UM-p400-HR-Thermo-3.pdf |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | NA |
| Solvent A: | 290mL Methanol + 1 ampule FIA mobile phase additive |
| Chromatography Type: | None (Direct infusion) |
| Chromatography ID: | CH002748 |
| Chromatography Summary: | FIA2 method for analysis of acylcarnitines For more details, please refer sections 4.6, 4.7, 5, 9, 10.1, and 10.2 of the method file attached. |
| Methods Filename: | UM-p400-HR-Thermo-3.pdf |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | NA |
| Solvent A: | 290mL Methanol + 1 ampule FIA mobile phase additive |
| Chromatography Type: | None (Direct infusion) |
