Summary of Study ST002231
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001421. The data can be accessed directly via it's Project DOI: 10.21228/M81X41 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002231 |
| Study Title | Metabolomics Analysis of HOG-EV and HOG-R132H Cells with and without BAY 2402234 Treatment |
| Study Type | Metabolomics Analysis |
| Study Summary | HOG cells were plated in 6-well plates (0.5 × 10^6 cells per well). 24 hours later, HOG-EV or HOG-R132H cells were treated for 24 hours with 10 nM BAY 2402234 or DMSO. Cells were then harvested for LC-MS analysis. |
| Institute | UT Southwestern Medical Center |
| Department | Children's Research Institute |
| Laboratory | McBrayer Laboratory |
| Last Name | McBrayer |
| First Name | Samuel |
| Address | 6000 Harry Hines Boulevard, NL10.110K, Dallas, TX 75235, USA |
| samuel.mcbrayer@utsouthwestern.edu | |
| Phone | (214)-648-3730 |
| Submit Date | 2022-07-14 |
| Num Groups | 4 |
| Total Subjects | 1 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2022-07-28 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003640 | AN003641 |
|---|---|---|
| Chromatography ID | CH002695 | CH002695 |
| MS ID | MS003391 | MS003392 |
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | Vanquish Flex UHPLC | Vanquish Flex UHPLC |
| Column | Millipore ZIC-pHILIC | Millipore ZIC-pHILIC |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive HF-X Orbitrap | Thermo Q Exactive HF-X Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | TIC-Corrected Peak Area | TIC-Corrected Peak Area |
Chromatography:
| Chromatography ID: | CH002695 |
| Chromatography Summary: | Chromatographic resolution of metabolites was achieved using on a Millipore ZIC-pHILIC column using a linear gradient of 10 mM ammonium formate pH 9.8 and acetonitrile. |
| Instrument Name: | Vanquish Flex UHPLC |
| Column Name: | Millipore ZIC-pHILIC |
| Flow Gradient: | linear gradient of 10 mM ammonium formate pH 9.8 and acetonitrile |
| Solvent A: | 100% water; 10 mM ammonium formate pH 9.8 |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | HILIC |
