Summary of Study ST002174

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001383. The data can be accessed directly via it's Project DOI: 10.21228/M8Z993 This work is supported by NIH grant, U2C- DK119886.


Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files (Contains raw data)
Study IDST002174
Study TitleIdentifying a tryptophan derivative in hydrogen peroxide-treated cell culture medium
Study SummaryReactive oxygen species (ROS) are by-products of metabolism of oxygen and they play an important role in normal homeostasis and cell signaling, as well as in the initiation of diseases including cancer when their production is upregulated. Thus, it is imperative to understand the cellular and molecular basis by which ROS impact on various biological and pathological processes. Here, we identified 2-oxindole, a tryptophan derivative, was a major catabolic product in hydrogen peroxide-treated cell culture medium. We used 2-oxindole to study its role in regulating AhR signaling and tryptophan metabolic pathways. We found that 2-oxindole significantly increased the activity of AhR, leading to enhanced expression of its downstream targets including cytochrome P450 genes.
NYU Langone Health
DepartmentDepartment of Biochemistry and Molecular Pharmacology
Last NameChoi
First NameByeong Hyeok
Address341 East 25 Street New York, NY 10010
Submit Date2022-04-06
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailLC-MS
Release Date2022-06-01
Release Version1
Byeong Hyeok Choi Byeong Hyeok Choi application/zip

Select appropriate tab below to view additional metadata details:

Combined analysis:

Analysis ID AN003562
Analysis type MS
Chromatography type HILIC
Chromatography system Ulitmate 3000
Column SeQuant ZIC-pHILIC (150 x 4.6mm,5um)
MS instrument type Triple quadrupole
MS instrument name Thermo Q Exactive Orbitrap
Units N/L


Chromatography ID:CH002633
Chromatography Summary:SeQuant ZIC-pHILIC 5um polymer, 150x2.1mm - Part No. 15046000001 Buffer A: 10mM ammonium carbonate in water, LCMS Grade Buffer B: Acetonitrile, LCMS Grade Rear Wash: 10% methanol in water, LCMS Grade Wash Buffer: water, LCMS Grade Injection Volume: 2uL
Methods Filename:L12
Instrument Name:Ulitmate 3000
Column Name:SeQuant ZIC-pHILIC (150 x 4.6mm,5um)
Column Pressure:1800
Column Temperature:25
Flow Rate:0.100ml/min
Injection Temperature:4
Internal Standard:ISTD (500nM amino acid cocktail)
Solvent A:100% water; 10 mM ammonium carbonate
Solvent B:100% acetonitrile
Chromatography Type:HILIC