Summary of Study ST001467
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000999. The data can be accessed directly via it's Project DOI: 10.21228/M8K10H This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001467 |
| Study Title | Metabolites changes related to glucose-mediated energy production in chemotheraphy-induced cachexia |
| Study Summary | Targeted metabolomics platforms included amino acids and metabolites related to glucose-mediated energy production. The targeted metabolome changed with chemotheraphy-induced cachexia, and the changes were reversed with potential treatment of the cachexia. .rdb files were included as raw data files where detailed information regarding MRM transitions and internal standards can be found. Several amino acids (Gly, Pro, Gln, Taurine) were analyzed after dilution because their peak intensities were too high. Thus their analysis was performed separately from other amino acids, and their rdb files were saved in separate rdb files. |
| Institute | Asan Medical Center; University of Ulsan |
| Last Name | Yoo |
| First Name | Hyun Ju |
| Address | 88, Olympic-ro, 43-gil, Songpa-gu, Seoul 05505, South Korea |
| yoohyunju@amc.seoul.kr | |
| Phone | 82-02-3010-4029 |
| Submit Date | 2020-08-18 |
| Raw Data Available | Yes |
| Analysis Type Detail | LC-MS |
| Release Date | 2020-09-21 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002443 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent 1290 |
| Column | Zorbax Eclipse XDB-C18 (100 x 2mm) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | ABI Sciex 5500 QTrap |
| Ion Mode | POSITIVE |
| Units | area ratio |
Chromatography:
| Chromatography ID: | CH001788 |
| Chromatography Summary: | Metabolites related to glucose-mediated metabolites were analyzed with LC-MS/MS (MRM) |
| Instrument Name: | Agilent 1290 |
| Column Name: | Synergi fusion RP (50 x 2mm) |
| Column Temperature: | 23 |
| Flow Gradient: | 0% B for 5 min, 0% to 90% B for 2 min, hold at 90% B for 8 min, 90% to 0% B for 1 min, and then hold at 0% B for 9 min |
| Flow Rate: | 70 μL/min except for minutes 7 to 15, when it was 140 µL/min |
| Internal Standard: | 13C5-glutamine |
| Sample Injection: | 3 uL |
| Solvent A: | 100% water; 5 mM ammonium acetate |
| Solvent B: | 100% acetonitrile; 5 mM ammonium acetate |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH001789 |
| Chromatography Summary: | Amino acids and bioamines were analyzed with LC-MS/MS (MRM) |
| Instrument Name: | Agilent 1290 |
| Column Name: | Zorbax Eclipse XDB-C18 (100 x 2mm) |
| Column Temperature: | 50 |
| Flow Gradient: | 0% B for 0.5 min, 0% to 95% B for 5 min, hold at 95% B for 1 min, 95% to 0% B for 0.5 min, then hold at 0% B for 2.5 min |
| Flow Rate: | 500 μL/min |
| Internal Standard: | 13C5-glutamine, serotonine-d4, dopamine-d4, tryptophan-d5, serine-d3, and lysine-d8 |
| Sample Injection: | 3 uL |
| Solvent A: | 100% water; 0.2% formic acid |
| Solvent B: | 100% acetonitrile; 0.2% formic acid |
| Chromatography Type: | Reversed phase |
