Summary of Study ST000118
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000106. The data can be accessed directly via it's Project DOI: 10.21228/M8RC75 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000118 |
| Study Title | Metabolomics analysis of multiple metabolic functions in the YjgF/YER057c/UK114 (Rid) protein family GCMS (part 2) |
| Study Type | wildtype vs knock-out |
| Study Summary | Metabolomics analysis of wild-type S. enterica, single RidA knock-out and triple Rid (ridA Rid2 Rid7) knock-out S.enterica cells |
| Institute | University of California, Davis |
| Department | Genome Center |
| Laboratory | Fiehn Laboratory |
| Last Name | ElBadawi-Sidhu |
| First Name | Mona |
| Address | 451 Health Sciences Drive, Davis, California 95616, USA |
| mmelbadawi@ucdavis.edu | |
| Submit Date | 2014-11-06 |
| Num Groups | 5 |
| Total Subjects | 6 |
| Study Comments | One sample missing (A6) |
| Raw Data Available | Yes |
| Raw Data File Type(s) | cdf |
| Uploaded File Size | 611 M |
| Analysis Type Detail | GC-MS |
| Release Date | 2014-11-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN000199 |
|---|---|
| Chromatography ID | CH000132 |
| MS ID | MS000162 |
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 6890N |
| Column | Restek corporation Rtx-5Sil MS |
| MS Type | EI |
| MS instrument type | GC-TOF |
| MS instrument name | Leco Pegasus III GC TOF |
| Ion Mode | POSITIVE |
| Units | Peak area |
Chromatography:
| Chromatography ID: | CH000132 |
| Chromatography Summary: | Untargeted GC MS |
| Methods Filename: | Data_Dictionary_Fiehn_laboratory_GCTOF_MS_primary_metabolism_09-27-2013_general.pdf |
| Instrument Name: | Agilent 6890N |
| Column Name: | Restek corporation Rtx-5Sil MS |
| Column Temperature: | 50-330oC |
| Flow Rate: | 1ml/min |
| Internal Standard: | fatty acid methyl esters |
| Sample Injection: | 0.5uL |
| Oven Temperature: | 50C for 1 min, then ramped at 20C min-1 to 330C, held constant for 5 min |
| Transferline Temperature: | 280C |
| Washing Buffer: | Ethyl Acetate |
| Sample Loop Size: | 30 m length x 0.25 mm internal diameter |
| Randomization Order: | Excel generated |
| Chromatography Type: | GC |
