Summary of Study ST003913
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002448. The data can be accessed directly via it's Project DOI: 10.21228/M86R8C This work is supported by NIH grant, U2C- DK119886. See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST003913 |
| Study Title | Altered Metabolomics and Inflammatory Transcriptomics in Human Bone Marrow Adipocytes After Acute High Calorie Diet and Acute Fasting |
| Study Type | Clinical Research |
| Study Summary | Expansion of bone marrow (BM) adipocytes has been linked to nutritional pressures, suggesting that BM is a dynamic compartment that responds to fluctuations in systemic nutritional availability to regulate osteogenesis and hematopoiesis. Here we investigated BM metabolism in response to acute overnutrition (high calorie diet; HCD) and calorie deprivation (fasting). Participants underwent a 10-day HCD followed by a two-week interval of an ad libitum diet and then underwent 10 days of fasting. BM adipocytes and sera were collected before and after each dietary intervention. Using comprehensive and integrated analyses, we characterized nutritional influences on BM adiposity. BM adipocytes after HCD showed an upregulation of FOXP3 (p < 0.0001), the transcription factor that controls the development of Tregs, which are critical in reducing inflammatory immune responses. After fasting, BM adipocytes had an upregulation of inflammatory genes (CP, CFH, and IGFBP3) (p < 0.0001). Proteomic analysis after HCD showed that BM serum had an upregulation of proteins related to an inflammatory/complement pathway (PROC, RBP4, and CFI). After fasting, in BM serum there was a significant downregulation of inflammatory/complement pathway proteins (C1QC and RBP4). Despite both interventions causing BM adipose tissue expansion, the mechanism for adipogenesis appears to be dependent on nutrient availability. After HCD, lipid-mediated signaling and lipid storage and lipid droplet biogenesis were significantly downregulated (p < 0.0001). In contrast, after fasting lipid-mediated signaling and lipid storage and lipid droplet biogenesis were significantly upregulated (p < 0.0001). Overall, our results demonstrate key differences in inflammatory response and lipid metabolism between HCD and fasting, despite a nearly identical BM adipose phenotype. Further analyses are needed to understand the effects nutritional pressures have on BM adipogenesis and immune responses. |
| Institute | MaineHealth Institute for Research |
| Department | Center for Molecular Medicine |
| Laboratory | Clifford Rosen |
| Last Name | Vary |
| First Name | Calvin |
| Address | 81 Research Dr. Scarborough ME |
| calvin.vary@mainehealth.org | |
| Phone | 2073968148 |
| Submit Date | 2025-04-16 |
| Num Groups | 8; Fasting-Pre, Fasting-Post, HCD-Pre, HCD-Post for peripheral blood serum (PB) and bone marrow serum (BM) |
| Total Subjects | n = 4 for each dietary phase (HCD and fasting) and timepoint (Pre and Post) |
| Study Comments | Samples from four participants were used from a total of 10 women and 13 men. The sex of these four participants is unknown. Only these samples were used because we had enough peripheral blood and bone marrow sera from each of these participants to perform paired lipidomic analyses on the High Calorie-Post and -Pre as well as the Fasting-Post and -Pre. This way direct comparisons can be made between the peripheral blood and bone marrow sera and the dietary phases. |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML, wiff |
| Analysis Type Detail | LC-MS/MS(Dir. Inf.) |
| Release Date | 2025-05-26 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN006425 | AN006426 |
|---|---|---|
| Chromatography ID | CH004874 | CH004874 |
| MS ID | MS006126 | MS006127 |
| Analysis type | MS | MS |
| Chromatography type | None (Direct infusion) | None (Direct infusion) |
| Chromatography system | none | none |
| Column | none | none |
| MS Type | ESI | ESI |
| MS instrument type | Triple quadrupole | Triple quadrupole |
| MS instrument name | ABI Sciex 4000 QTrap | ABI Sciex 4000 QTrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | intensity | intensity |
