Summary of Study ST002826

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001768. The data can be accessed directly via it's Project DOI: 10.21228/M85T5M This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Show all samples  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files (Contains raw data)
Study IDST002826
Study TitleCold-stimulated brown adipose tissue activation is related to changes in serum metabolites relevant to NAD+ metabolism in humans
Study SummaryCold-induced brown adipose tissue (BAT) activation is considered to improve metabolic health. In murine BAT, cold increases the fundamental molecule for mitochondrial function, nicotinamide adenine dinucleotide (NAD+), but limited knowledge on NAD+ metabolism during cold in human BAT metabolism exists. We show that cold increases the serum metabolites of the NAD+ salvage pathway (nicotinamide and 1-methylnicotinamide) in humans. Additionally, individuals with coldstimulated BAT activation have decreased levels of metabolites from the de novo NAD+ biosynthesis pathway (tryptophan, kynurenine). Serum nicotinamide correlates positively with cold-stimulated BAT activation, whereas tryptophan and kynurenine correlates negatively. Furthermore, the expression of genes involved in NAD+ biosynthesis in BAT are related to markers of metabolic health. Our data indicate that cold increases serum tryptophan conversion to nicotinamide to be further utilized by BAT. We conclude that NAD+ metabolism is activated upon cold in humans and probably regulated in a coordinated fashion by several tissues.
Institute
University of Turku
Last NameVirtanen
First NameKirsi
AddressTurku PET Centre, Turku University Hospital, Turku, Finland
Emailkirsi.virtanen@utu.fi
Phone+358-40-7626564
Submit Date2023-08-10
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2023-09-11
Release Version1
Kirsi Virtanen Kirsi Virtanen
https://dx.doi.org/10.21228/M85T5M
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Combined analysis:

Analysis ID AN004610 AN004611 AN004612 AN004613
Analysis type MS MS MS MS
Chromatography type HILIC HILIC Reversed phase Reversed phase
Chromatography system Agilent 1290 Agilent 1290 Agilent 1290 Agilent 1290
Column Acquity UPLC BEH Amide column, 2.1 × 100 mm, 1.7 μm Acquity UPLC BEH Amide column, 2.1 × 100 mm, 1.7 μm Agilent Zorbax Eclipse XDB-C18 Rapid Resolution HD 1.8 μm, 2.1 x 100 mm Agilent Zorbax Eclipse XDB-C18 Rapid Resolution HD 1.8 μm, 2.1 x 100 mm
MS Type ESI ESI ESI ESI
MS instrument type QTOF QTOF QTOF QTOF
MS instrument name Agilent 6540 QTOF Agilent 6540 QTOF Agilent 6540 QTOF Agilent 6540 QTOF
Ion Mode POSITIVE NEGATIVE POSITIVE NEGATIVE
Units Area Area Area Area
  logo