Summary of Study ST002270
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001452. The data can be accessed directly via it's Project DOI: 10.21228/M81Q5B This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002270 |
Study Title | Xenopus tropicalis glycolysis and PPP inhibition |
Study Summary | Stage 41 tadpoles were injected with 4 nmol of the glycolysis inhibitor, 2-deoxyglucose (2DG), or a tracer control to evaluate consequences of inhibition on metabolites 24 hours after treatment began. Similarly, tadpoles were incubated in DMSO control or 1 of 2 G6PD inhibitors (Dehydroepiandrosterone and g6pdi), to similarly assess the consequences of inhibiting the pentose phosphate pathway. We find that inhibition of glucose metabolism with 2DG results in a decrease in downstream glycolytic intermediates, confirming a reduction in activity of this pathway. G6PD inhibition was not as clear as changes were less consistent across treatments and downstream metabolites did not behave in a coordinated way, though impacts on other metabolic processes by these inhibitors may be fruitful for exploring how they perturb metabolism in the tadpoles. |
Institute | University of Washington |
Last Name | Patel |
First Name | Jeet |
Address | 1705 NE Pacific St., HSB J-Wing, J405, Seattle, Washington, 98195, USA |
pateljeet1224@gmail.com | |
Phone | 2065431748 |
Submit Date | 2022-08-03 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzML |
Analysis Type Detail | LC-MS |
Release Date | 2022-10-25 |
Release Version | 1 |
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Combined analysis:
Analysis ID | AN003710 | AN003711 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | HILIC | HILIC |
Chromatography system | Shimadzu Nexera X2 | Shimadzu Nexera X2 |
Column | Waters XBridge BEH Amide XP | Waters XBridge BEH Amide XP |
MS Type | ESI | ESI |
MS instrument type | Triple quadrupole | Triple quadrupole |
MS instrument name | ABI Sciex 6500+ QTrap | ABI Sciex 6500+ QTrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | Peak Intensity | Peak intensity |