Summary of Study ST002174

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001383. The data can be accessed directly via it's Project DOI: 10.21228/M8Z993 This work is supported by NIH grant, U2C- DK119886.

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Study IDST002174
Study TitleIdentifying a tryptophan derivative in hydrogen peroxide-treated cell culture medium
Study SummaryReactive oxygen species (ROS) are by-products of metabolism of oxygen and they play an important role in normal homeostasis and cell signaling, as well as in the initiation of diseases including cancer when their production is upregulated. Thus, it is imperative to understand the cellular and molecular basis by which ROS impact on various biological and pathological processes. Here, we identified 2-oxindole, a tryptophan derivative, was a major catabolic product in hydrogen peroxide-treated cell culture medium. We used 2-oxindole to study its role in regulating AhR signaling and tryptophan metabolic pathways. We found that 2-oxindole significantly increased the activity of AhR, leading to enhanced expression of its downstream targets including cytochrome P450 genes.
Institute
NYU Langone Health
DepartmentDepartment of Biochemistry and Molecular Pharmacology
LaboratoryDai
Last NameChoi
First NameByeong Hyeok
Address341 East 25 Street New York, NY 10010
EmailByeongHyeok.Choi@nyulangone.org
Phone2122635521
Submit Date2022-04-06
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailLC-MS
Release Date2022-06-01
Release Version1
Byeong Hyeok Choi Byeong Hyeok Choi
https://dx.doi.org/10.21228/M8Z993
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN003562
Analysis type MS
Chromatography type HILIC
Chromatography system Ulitmate 3000
Column SeQuant ZIC-pHILIC 5um polymer, 150x2.1mm
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Thermo Q Exactive Orbitrap
Ion Mode UNSPECIFIED
Units N/L
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