Summary of Study ST002569

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001656. The data can be accessed directly via it's Project DOI: 10.21228/M8ND9B This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002569
Study TitleLipidomic analysis in very-long chain acyl-CoA dehydrogenase null mice
Study SummaryCompared to their littermate counterparts, HFD/fasted VLCAD-/- mouse hearts displayed higher VLC-acylcarnitines accumulation, higher levels of arachidonic acid (AA) and lower docosahexaenoic acid (DHA) contents in glycerophospholipids (GPLs).
Montreal Heart Institute
Last NameForest
First NameAnik
Address5000 Bélanger Street
Phone5143763330 ext 2133
Submit Date2023-04-11
Analysis Type DetailLC-MS
Release Date2023-09-06
Release Version1
Anik Forest Anik Forest application/zip

Select appropriate tab below to view additional metadata details:


Project ID:PR001656
Project DOI:doi: 10.21228/M8ND9B
Project Title:Remodeling of lipid landscape in high fat fed very-long chain acyl-CoA dehydrogenase null mice favors pro-arrhythmic polyunsaturated fatty acids and their downstream metabolites
Project Summary:Very-long chain acyl-CoA dehydrogenase (VLCAD) catalyses mitochondrial fatty acid β-oxidation (FAO). Inherited VLCAD deficiency (VLCADD) predisposes to neonatal arrhythmias whose pathophysiology is still not understood. We hypothesized that VLCADD results in global disruption of cardiac complex lipid homeostasis, which may set conditions predisposing to arrhythmia. To test this, we assessed the cardiac lipidome and related molecular markers in seven-month-old VLCAD-/- mice, which mimic to some extent the human cardiac phenotype. Mice were sacrificed in the fed or fasted state after receiving for two weeks a chow or a high-fat diet (HFD), the latter condition being known to worsen symptoms in human VLCADD. Compared to their littermate counterparts, HFD/fasted VLCAD-/- mouse hearts displayed the following lipid alterations: (1) Lower LC, but higher VLC-acylcarnitines accumulation, (2) higher levels of arachidonic acid (AA) and lower docosahexaenoic acid (DHA) contents in glycerophospholipids (GPLs), as well as (3) corresponding changes in pro-arrhythmogenic AA-derived isoprostanes and thromboxane B2 (higher), and anti-arrythmogenic DHA-derived neuroprostanes (lower). These changes were associated with remodeling in the expression of gene or protein markers of (1) PL remodeling: higher calcium-dependent phospholipase A2 and lysophosphatidylcholine-acyltransferase 2, (2) calcium handling perturbations: higher SERCA2a and phospholamban, but lower RyR2, and (3) endoplasmic reticulum stress: higher CHOP and GRP78. Altogether, these results highlight global lipid dyshomeostasis beyond FAO in VLCAD-/- mouse hearts, which may set conditions predisposing the hearts to calcium mishandling and endoplasmic reticulum stress and thereby may contribute to the pathogenesis of arrhythmias in VLCADD in mice as well as in humans.
Institute:Montreal Heart Institute
Last Name:Forest
First Name:Anik
Address:5000 Bélanger Street
Phone:5143763330 ext 2133


Subject ID:SU002670
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090


Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Diet Nutritional status Genotype
SA2582655973High Fat diet Fasted VLCAD -/-
SA2582665974High Fat diet Fasted VLCAD -/-
SA2582675972High Fat diet Fasted VLCAD -/-
SA2582685971High Fat diet Fasted VLCAD -/-
SA2582695970High Fat diet Fasted VLCAD -/-
SA2582705975High Fat diet Fasted VLCAD -/-
SA2582715976High Fat diet Fasted VLCAD -/-
SA2582725980High Fat diet Fasted VLCAD -/-
SA2582735978High Fat diet Fasted VLCAD -/-
SA2582745979High Fat diet Fasted VLCAD -/-
SA2582755977High Fat diet Fasted VLCAD -/-
SA2582555964High Fat diet Fasted VLCAD +/+
SA2582565963High Fat diet Fasted VLCAD +/+
SA2582575962High Fat diet Fasted VLCAD +/+
SA2582585965High Fat diet Fasted VLCAD +/+
SA2582595967High Fat diet Fasted VLCAD +/+
SA2582605969High Fat diet Fasted VLCAD +/+
SA2582615968High Fat diet Fasted VLCAD +/+
SA2582625959High Fat diet Fasted VLCAD +/+
SA2582635966High Fat diet Fasted VLCAD +/+
SA2582645961High Fat diet Fasted VLCAD +/+
SA2582855701Standard diet Fed VLCAD -/-
SA2582865699Standard diet Fed VLCAD -/-
SA2582875702Standard diet Fed VLCAD -/-
SA2582885700Standard diet Fed VLCAD -/-
SA2582895704Standard diet Fed VLCAD -/-
SA2582905696Standard diet Fed VLCAD -/-
SA2582915698Standard diet Fed VLCAD -/-
SA2582925697Standard diet Fed VLCAD -/-
SA2582935703Standard diet Fed VLCAD -/-
SA2582765689Standard diet Fed VLCAD +/+
SA2582775688Standard diet Fed VLCAD +/+
SA2582785690Standard diet Fed VLCAD +/+
SA2582795691Standard diet Fed VLCAD +/+
SA2582805695Standard diet Fed VLCAD +/+
SA2582815694Standard diet Fed VLCAD +/+
SA2582825687Standard diet Fed VLCAD +/+
SA2582835693Standard diet Fed VLCAD +/+
SA2582845692Standard diet Fed VLCAD +/+
Showing results 1 to 39 of 39


Collection ID:CO002663
Collection Summary:Mice were sacrificed under anesthesia using ketamine (100mg/ml) and xylazine (20mg/ml). Immediately after mouse sacrifice, hearts were rapidly excised, snap-frozen with liquid nitrogen, and then stored at -80°C.
Sample Type:Heart
Storage Conditions:-80℃
Collection Tube Temp:-80


Treatment ID:TR002682
Treatment Summary:VLCAD-/- males (129svJ X C57BL/6J genetic background) were crossed with C57Bl/6J females to generate heterozygous VLCAD+/- mice. Coupling of heterozygous mice then produced VLCAD-/- mice and control littermate counterparts (VLCAD+/+). All animals were genotyped as previously described. Mice were housed in a specific pathogen-free facility under a 12-h light/dark cycle at constant temperature and with access to water and food ad libitum. At 7-month of age, mice were fed for 2 weeks with either a chow diet (Harlan no. 2018; 3.1 kcal/g) or a high-fat diet (Harlan Teklab no. 3584; 5.4kcal/g) ad libitum. Mice were sacrificed in fed state for the CD group (CD/fed condition) or after fasting for 24 hours for the HFD group (HFD/fasted condition. Percent calories from carbohydrates, lipids and proteins were 58/18/24 for the CD and 26.6/58.4/15 for the HFD.

Sample Preparation:

Sampleprep ID:SP002676
Sampleprep Summary:Total lipids were extracted from 40mg of pulverized hearts under liquid nitrogen using in methyl tert-butyl ether (MTBE) and ethyl acetate after spiking with the following internal standards lysophosphatidylcholine (LPC) 13:0, phosphatidylcholine (PC) 19:0/19:0, PC14:0/14:0, phosphatidylserine (PS) 12:0/12:0, phosphatidylglycerol (PG) 15:0/15:0 and phosphatidylethanolamine (PE) 17:0/17:0). Supernatants were evaporated using a Speed Vacuum concentrator overnight, dissolved in methanol/chloroform (2:1) and aliquots (50 µL) were stored at -80ºC until use.
Sampleprep Protocol Filename:Sample preparation protocol of untargeted lipidomic analysis
Processing Storage Conditions:-80℃

Combined analysis:

Analysis ID AN004233
Analysis type MS
Chromatography type Reversed phase
Chromatography system Agilent 1290 Infinity
Column Agilent ZORBAX Eclipse Plus C18 (100 x 2.1mm,1.8um)
MS instrument type QTOF
MS instrument name Agilent 6530 QTOF
Units Counts


Chromatography ID:CH003141
Methods Filename:LC-MS and data processing protocol of untargeted lipidomic analysis
Instrument Name:Agilent 1290 Infinity
Column Name:Agilent ZORBAX Eclipse Plus C18 (100 x 2.1mm,1.8um)
Column Temperature:40
Flow Gradient:0–2 min, 50% B; 2–11 min, 50–85% B; 11–33 min, 85–94% B; 33–35 min, 94–98% B, 35–38 min; 98–99.5% B; 38–82 min, 98–99.5% B; 82–83 min, 99.5–50% B, plus an equilibration of 8 min
Flow Rate:0.45 ml/min
Solvent A:100% water; 0.2% formic acid; 10 mM ammonium formate
Solvent B:55% ethanol,/35% acetonitrile/10% MTBE, 55:35:10; 0.2% formic acid; 10 mM ammonium formate
Chromatography Type:Reversed phase


MS ID:MS003980
Analysis ID:AN004233
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Comments:MS data processing was achieved using the Mass Hunter Qualitative Analysis software package (version B.07) for peak picking and a bioinformatic script that we have developed and encoded in both Perl and R languages to enable optimal MS data alignment, filtering of presence, normalisation, batch effect correction, and missing data imputation. The resulting final dataset list reproducible high quality MS signals referred as features with their mass-over-charge (m/z), corrected signal intensity, and retention time (RT). Feature identity (ID) was as following: ionization mode:mass@RT.