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MB Sample ID: SA300839
Local Sample ID: | HPLT_S1 |
Subject ID: | SU002911 |
Subject Type: | Bacteria |
Subject Species: | Microbacterium sediminis YLB-01 |
Species Group: | Bacteria |
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Treatment:
Treatment ID: | TR002920 |
Treatment Summary: | To ensure an adequate cell count, YLB-01 cultures were initially cultivated under optimal conditions (28°C, 0.1 MPa) for 24 h. The optical density at 600 nm (OD600 nm) of the cell cultures was measured using an automatic growth curve analyzer (FP-1100-C, Growth Curves Ab Ltd., Finland). Once the cultures reached the stationary phase of growth, the cells were transferred into a 100-mL sterile saline bag and placed in a high-pressure culture kettle. The cells were then exposed to either 30 MPa (the HPLT group, N=8) or 0.1 MPa (the NPLT group, N=7) at 4°C for 7 days. |