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MB Sample ID: SA258257
Local Sample ID: | 5962 |
Subject ID: | SU002670 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Gender: | Male |
Species Group: | Mammals |
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Treatment:
Treatment ID: | TR002682 |
Treatment Summary: | VLCAD-/- males (129svJ X C57BL/6J genetic background) were crossed with C57Bl/6J females to generate heterozygous VLCAD+/- mice. Coupling of heterozygous mice then produced VLCAD-/- mice and control littermate counterparts (VLCAD+/+). All animals were genotyped as previously described. Mice were housed in a specific pathogen-free facility under a 12-h light/dark cycle at constant temperature and with access to water and food ad libitum. At 7-month of age, mice were fed for 2 weeks with either a chow diet (Harlan no. 2018; 3.1 kcal/g) or a high-fat diet (Harlan Teklab no. 3584; 5.4kcal/g) ad libitum. Mice were sacrificed in fed state for the CD group (CD/fed condition) or after fasting for 24 hours for the HFD group (HFD/fasted condition. Percent calories from carbohydrates, lipids and proteins were 58/18/24 for the CD and 26.6/58.4/15 for the HFD. |