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MB Sample ID: SA229301
Local Sample ID: | 191115_FW_1_project_Ermolaeva_phosphoPC014 |
Subject ID: | SU002411 |
Subject Type: | Invertebrate |
Subject Species: | Caenorhabditis elegans |
Taxonomy ID: | 6239 |
Age Or Age Range: | Young, middle aged and old |
Species Group: | Invertebrates |
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Treatment:
Treatment ID: | TR002423 |
Treatment Summary: | Ncl-1 and tif-IA knockdown was achieved by feeding the worms for 2 generations with bacteria (from the Ahringer RNAi feeding library, Source BioScience Ltd.) producing the corresponding dsRNA. Worms were cultured under standard conditions as described in ‘Wormbook’, F1 was grown at 15°C and F2 at 20°C, and F2 animals were grown on RNAi until days 2, 6 or 12 of adulthood. |