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MB Sample ID: SA229289

Local Sample ID:191115_FW_1_project_Ermolaeva_1_10_PS040
Subject ID:SU002411
Subject Type:Invertebrate
Subject Species:Caenorhabditis elegans
Taxonomy ID:6239
Age Or Age Range:Young, middle aged and old

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Treatment ID:TR002423
Treatment Summary:Ncl-1 and tif-IA knockdown was achieved by feeding the worms for 2 generations with bacteria (from the Ahringer RNAi feeding library, Source BioScience Ltd.) producing the corresponding dsRNA. Worms were cultured under standard conditions as described in ‘Wormbook’, F1 was grown at 15°C and F2 at 20°C, and F2 animals were grown on RNAi until days 2, 6 or 12 of adulthood.